Chen Kangwu, Mo Jianqiang, Zhou Ming, Wang Genlin, Wu Guizhong, Chen Hao, Zhang Kai, Yang Huilin
Department of Orthopedic Surgery, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou, 215006, Jiangsu, China.
Med Oncol. 2014 Apr;31(4):886. doi: 10.1007/s12032-014-0886-7. Epub 2014 Feb 18.
Sacral chordoma is an aggressive, locally invasive neoplasm, and has a poor prognosis. However, the molecular basis for the clinical behavior remains unknown. The purpose of this study was to investigate the expression of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) and mammalian target of rapamycin (mTOR) in sacral chordoma, and explore their roles in the prognosis. PTEN and mTOR were detected immunohistochemically in 40 sacral chordoma tissues and 10 adjacent normal tissues. Correlations between PTEN and mTOR expression and clinicopathological factors were analyzed. Kaplan-Meier survival curves and log-rank test were used to analyze the continuous disease-free survival time (CDFS). The expression of PTEN in sacral chordoma was significantly lower than that in adjacent normal tissues, while the levels of mTOR expression in sacral chordoma were significantly higher than that in adjacent normal tissues (P = 0.000, P = 0.030). The positive expression of mTOR appears to correlate with the negative expression of PTEN in sacral chordoma (P = 0.021). PTEN-negative expression and mTOR-positive expression were associated with tumor invasion into the surrounding muscles (P = 0.038, P = 0.014). Log-rank test showed that PTEN-negative and mTOR-positive expressions had an important impact on the patients' CDFS (P = 0.011, P = 0.015). Our results suggest that PTEN and mTOR might play an important role in the local invasiveness of sacral chordoma. PTEN and mTOR might be recognized as important prognostic predictors of recurrence and could be used as potential therapeutic targets for the treatment for sacral chordoma.
骶骨脊索瘤是一种侵袭性、局部浸润性肿瘤，预后较差。然而，其临床行为的分子基础仍不清楚。本研究旨在探讨10号染色体缺失的磷酸酶和张力蛋白同源物（PTEN）及雷帕霉素哺乳动物靶蛋白（mTOR）在骶骨脊索瘤中的表达，并探讨它们在预后中的作用。采用免疫组织化学方法检测40例骶骨脊索瘤组织和10例相邻正常组织中PTEN和mTOR的表达。分析PTEN和mTOR表达与临床病理因素之间的相关性。采用Kaplan-Meier生存曲线和对数秩检验分析无病生存时间（CDFS）。骶骨脊索瘤中PTEN的表达明显低于相邻正常组织，而骶骨脊索瘤中mTOR的表达水平明显高于相邻正常组织（P = 0.000，P = 0.030）。在骶骨脊索瘤中，mTOR的阳性表达似乎与PTEN的阴性表达相关（P = 0.021）。PTEN阴性表达和mTOR阳性表达与肿瘤侵犯周围肌肉有关（P = 0.038，P = 0.014）。对数秩检验显示，PTEN阴性和mTOR阳性表达对患者的CDFS有重要影响（P = 0.011，P = 0.015）。我们的结果表明，PTEN和mTOR可能在骶骨脊索瘤的局部浸润中起重要作用。PTEN和mTOR可能被认为是复发的重要预后预测指标，并可作为骶骨脊索瘤治疗的潜在治疗靶点。
