从土耳其科鲁河谷采集的榅桲（Cydonia oblonga Mill.）基因型之间的遗传变异。

Genetic variation among quince (Cydonia oblonga Mill.) genotypes sampled from the Coruh valley in Turkey.

作者信息

Orhan E, Nardemir G, Agar G, Ercisli S

机构信息

Department of Agricultural Biotechnology, Faculty of Agriculture, Erzurum, Turkey.

Department of Biology, Faculty of Sciences, Ataturk University, Erzurum, Turkey.

出版信息

Genet Mol Res. 2014 Jan 21;13(1):445-9. doi: 10.4238/2014.January.21.12.

DOI:10.4238/2014.January.21.12

PMID:24535871

Abstract

Turkey has very rich quince genetic resources, and the country currently dominates world quince production. In particular, the northeastern part of the country has notable Cydonia oblonga Mill. germplasm. Authenticating the identity of germplasm resources of C. oblonga Mill. would be of great value for breeding practices. In the present study, genetic variations of 14 C. oblonga Mill. genotypes sampled from the Coruh valley of Turkey were investigated. Ten random primers generated 53 DNA markers. The highest polymorphism ratio was observed in the OPA07 primer (86%), while the lowest was observed in OPA03 (29%). The percentage of polymorphic bands was 51%, which demonstrated the efficiency of the primers used. The similarity matrix revealed that the similarity among genotypes ranged between 0.42 and 0.96. The identified random amplified polymorphic DNA markers enabled clear discrimination among all genotypes considered.

摘要

土耳其拥有非常丰富的榅桲遗传资源，该国目前在世界榅桲生产中占据主导地位。特别是该国东北部拥有显著的榅桲（Cydonia oblonga Mill.）种质资源。鉴定榅桲（C. oblonga Mill.）种质资源的身份对于育种实践具有重要价值。在本研究中，对从土耳其乔鲁赫山谷采集的14个榅桲（C. oblonga Mill.）基因型的遗传变异进行了研究。10条随机引物产生了53个DNA标记。在OPA07引物中观察到最高的多态性比率（86%），而在OPA03中观察到最低的多态性比率（29%）。多态性条带的百分比为51%，这证明了所用引物的有效性。相似性矩阵显示，基因型之间的相似性在0.42至0.96之间。所鉴定的随机扩增多态性DNA标记能够对所有考虑的基因型进行清晰区分。