利用SSR标记揭示土耳其野生甜樱桃（Prunus avium）的遗传多样性。

Genetic diversity in wild sweet cherries (Prunus avium) in Turkey revealed by SSR markers.

作者信息

Ercisli S, Agar G, Yildirim N, Duralija B, Vokurka A, Karlidag H

机构信息

Department of Horticulture, Faculty of Agriculture, Ataturk University, Erzurum, Turkey.

出版信息

Genet Mol Res. 2011 Jun 21;10(2):1211-9. doi: 10.4238/vol10-2gmr1196.

DOI:10.4238/vol10-2gmr1196

PMID:21732285

Abstract

Wild sweet cherry (Prunus avium) trees are abundant in the northern part of Turkey, including the Coruh Valley. We analyzed 18 wild sweet cherry genotypes collected from diverse environments in the upper Coruh Valley in Turkey to determine genetic variation, using 10 SSR primers. These SSR primers generated 46 alleles; the number of alleles per primer ranged from 3 to 7, with a mean of 4.6. The primer PS12A02 gave the highest number of polymorphic bands (N = 7), while CPSCT010, UDAp-401 and UDAp-404 gave the lowest number (N = 3). Seven groups were separated in the dendrogram, although most of the genotypes did not cluster according to phenological and morphological traits. This level of genetic diversity in these wild sweet cherry genotypes is very high and therefore these trees would be useful as breeders for crosses between cultivated sweet cherry and wild genotypes.

摘要

野生甜樱桃（Prunus avium）树在土耳其北部很常见，包括乔鲁赫河谷。我们使用10对SSR引物，对从土耳其乔鲁赫河上游不同环境中收集的18个野生甜樱桃基因型进行了分析，以确定其遗传变异。这些SSR引物产生了46个等位基因；每个引物的等位基因数量在3到7个之间，平均为4.6个。引物PS12A02产生的多态性条带数量最多（N = 7），而CPSCT010、UDAp - 401和UDAp - 404产生的数量最少（N = 3）。在聚类图中分离出了7组，不过大多数基因型并未根据物候和形态特征聚类。这些野生甜樱桃基因型的这种遗传多样性水平非常高，因此这些树对于培育甜樱桃与野生基因型之间的杂交品种将很有用。