An effective method for extracting total RNA from Dioscorea opposita Thunb.
作者信息
Liao C M, Li J, Liu X H, Zhang Y S
机构信息
Library, China West Normal University, Nanchong City, China.
College of Life Science, China West Normal University, Nanchong City, China.
出版信息
Genet Mol Res. 2014 Jan 21;13(1):462-8. doi: 10.4238/2014.January.21.15.
PMID:24535874
Abstract
Dioscorea opposita Thunb., included in the genus Dioscorea of the family Dioscoreaceae, is an important herb with great edible and medicinal value. In this study, the total RNA from leaves of Lichuan Dioscorea opposita Thunb. was isolated by an improved Trizol method. The results showed that the RNA extracted by the improved Trizol method had good integrity, and the RNA could be used for down-stream molecular biology operations including reverse transcription-polymerase chain reaction.
摘要
Zotero 插件