Proctor G B, Horobin R W
Department of Oral Pathology, Kings College School of Medicine and Dentistry, London, England.
Stain Technol. 1988 Mar;63(2):101-11. doi: 10.3109/10520298809107169.
Chemical properties of Weigert's resorcin-fuchsin, orcinol-new fuchsin, Sheridan's crystal violet and their resorcinol-free analogues were investigated using reverse-phase and gel filtration chromatography, electrophoresis, and visible light spectroscopy. Their staining properties were also studied. It was concluded that 1) the staining components of Weigert's resorcin-fuchsin, orcinol-new fuchsin and their resorcinol-free analogues are all indamine oligomers, 2) resorcinol is required for the production of Sheridan's crystal violet, the staining components of which consist of crystal violet substituted by varying numbers of resorcinyl substituents, 3) the staining components of all preparations are cationic (i.e., basic) dyes, 4) iron is present in staining solutions as the tetrachloroferrate anion (FeCl4-) and not as Fe or as a dye-chelate, and 5) since even the smallest Weigert's resorcin-fuchsin, orcinol-new fuchsin or Sheridan's crystal violet component has a conjugated bond number of 32, the observed staining of elastic fibers is only as expected.
利用反相和凝胶过滤色谱法、电泳法以及可见光光谱法研究了魏格特间苯二酚品红、地衣酚新品红、谢里丹结晶紫及其无间苯二酚类似物的化学性质。还研究了它们的染色特性。得出以下结论：1）魏格特间苯二酚品红、地衣酚新品红及其无间苯二酚类似物的染色成分均为吲达胺低聚物；2）谢里丹结晶紫的生产需要间苯二酚，其染色成分由被不同数量间苯二酚取代基取代的结晶紫组成；3）所有制剂的染色成分均为阳离子（即碱性）染料；4）染色溶液中的铁以四氯铁酸根阴离子（FeCl4-）形式存在，而非以铁或染料螯合物形式存在；5）由于即使是最小的魏格特间苯二酚品红、地衣酚新品红或谢里丹结晶紫成分的共轭键数也为32，因此观察到的弹性纤维染色是符合预期的。
