Angus B, Kiberu S, Purvis J, Wilkinson L, Horne C H
University Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne, U.K.
J Pathol. 1988 May;155(1):71-5. doi: 10.1002/path.1711550111.
The expression of low molecular weight cytokeratins (normally expressed only in simple epithelia) in intraepithelial neoplasia of grade CIN III or greater in cervical biopsies has recently been described by Bobrow et al. Our study of 127 cases confirms this finding and in addition we compare the use of three monoclonal antibodies, namely NCL-5D3, CAM 5.2, and PKK1, in demonstrating the phenomenon. Both NCL-5D3 and CAM 5.2 give consistently negative results for non-neoplastic stratified squamous epithelium, as well as CIN I and CIN II lesions, whilst staining about 30 per cent of CIN III biopsies and most carcinomas. PKK1, on the other hand, stained 50 per cent of non-neoplastic epithelia and thus did not serve as a marker of severe dysplasia. The possible implications of these observations are discussed.
低分子量细胞角蛋白（通常仅在单层上皮中表达）在宫颈活检中CIN III级及以上的上皮内瘤变中的表达，最近已由Bobrow等人进行了描述。我们对127例病例的研究证实了这一发现，此外，我们比较了三种单克隆抗体，即NCL - 5D3、CAM 5.2和PKK1在显示该现象中的应用。NCL - 5D3和CAM 5.2对非肿瘤性复层鳞状上皮以及CIN I和CIN II病变始终给出阴性结果，而对约30%的CIN III活检组织和大多数癌组织呈阳性染色。另一方面，PKK1对50%的非肿瘤性上皮呈阳性染色，因此不能作为重度发育异常的标志物。讨论了这些观察结果可能的意义。
