Pearson J C, Jennes L
Department of Anatomy, School of Medicine, Wright State University, Dayton, OH 45435.
Neurosci Lett. 1988 May 26;88(2):151-6. doi: 10.1016/0304-3940(88)90117-6.
A double immunofluorescence labeling method was used to study the localization of serotonin (5-HT)- and substance P (SP)-like immunoreactivities within neuronal fibers in the caudal spinal trigeminal nucleus of the rat. The 5-HT- and SP-immunoreactive fibers share extensive topographical overlap within the superficial laminae of the caudal trigeminal nucleus; however, the majority of stained fibers are immunoreactive for either 5-HT or SP, but not both simultaneously. A small population of fibers in which 5-HT and SP are co-localized is present and restricted to the marginal zone as well as the inner and outer layers of the substantia gelatinosa. The results of the present study suggest that fibers containing coexistent 5-HT and SP may be involved in the processing of nociceptive somatic sensation in the medullary dorsal horn of rat.
采用双重免疫荧光标记法研究大鼠尾侧三叉神经脊束核神经元纤维中5-羟色胺（5-HT）和P物质（SP）样免疫反应性的定位。5-HT和SP免疫反应性纤维在尾侧三叉神经核浅层有广泛的地形重叠；然而，大多数染色纤维对5-HT或SP呈免疫反应性，但不同时对两者呈免疫反应性。存在一小部分5-HT和SP共定位的纤维，且局限于边缘区以及胶状质的内层和外层。本研究结果表明，同时含有5-HT和SP的纤维可能参与大鼠延髓背角伤害性躯体感觉的处理。
