Nachbaur K, Troppmair J, Bieling P, Kotlan B, König P, Huber C
Division of Clinical Immunobiology, University Hospital, Innsbruck, Austria.
Immunobiology. 1988 Apr;177(1):55-65. doi: 10.1016/s0171-2985(88)80091-3.
The role of cytokines in the control of beta-2 microglobulin release from various haemopoietic cells was studied in vitro. Cell types investigated were resting cells or blasts of the T cell, B cell or monocyte-macrophage lineage. Mediators used in these experiments were r-IFN-alpha 2c, r-IFN-gamma, r-TNF-alpha, r-TNF-beta, r-IL1, r-IL2 and r-GM-CSF. Nanogram amounts of some of these mediators strongly affected beta-2 microglobulin release in vitro. r-IFN-gamma, r-IFN-alpha 2c and r-IL2 strongly enhanced and r-TNF-alpha and r-TNF-beta occasionally increased shedding of beta-2 microglobulin. r-IL1 and high concentrations of r-IFN-alpha 2c were inhibitory, whereas r-GM-CSF was ineffective. The impact of antigenic stimulation on beta-2 microglobulin release in mixed lymphocyte culture (MLC) was also studied. Stimulation with alloantigens in MLC greatly enhanced beta-2 microglobulin shedding, and this enhancement could be inhibited by a monoclonal antibody neutralizing human IFN-gamma. Among the various cell types studied, macrophages derived from peripheral blood monocytes were most susceptible to cytokine-induced alterations of beta-2 microglobulin shedding. From these data, we conclude that cytokines not only control the static expression of beta-2 microglobulin on the surface of haemopoietic cells but also largely affect their shedding.
体外研究了细胞因子在控制β2微球蛋白从各种造血细胞释放中的作用。所研究的细胞类型包括T细胞、B细胞或单核细胞-巨噬细胞系的静息细胞或母细胞。这些实验中使用的介质有重组α-干扰素2c（r-IFN-α2c）、重组γ-干扰素（r-IFN-γ）、重组α-肿瘤坏死因子（r-TNF-α）、重组β-肿瘤坏死因子（r-TNF-β）、重组白细胞介素1（r-IL1）、重组白细胞介素2（r-IL2）和重组粒细胞-巨噬细胞集落刺激因子（r-GM-CSF）。其中一些介质的纳克量在体外强烈影响β2微球蛋白的释放。r-IFN-γ、r-IFN-α2c和r-IL2强烈增强，而r-TNF-α和r-TNF-β偶尔会增加β2微球蛋白的脱落。r-IL1和高浓度的r-IFN-α2c具有抑制作用，而r-GM-CSF则无效。还研究了抗原刺激对混合淋巴细胞培养（MLC）中β2微球蛋白释放的影响。MLC中同种异体抗原刺激极大地增强了β2微球蛋白的脱落，并且这种增强可以被中和人IFN-γ的单克隆抗体抑制。在所研究的各种细胞类型中，外周血单核细胞来源的巨噬细胞对细胞因子诱导的β2微球蛋白脱落变化最为敏感。从这些数据中，我们得出结论，细胞因子不仅控制造血细胞表面β2微球蛋白的静态表达，而且在很大程度上影响其脱落。
