Butscher W G, Faubert G M
Institute of Parasitology, McGill University, Macdonald College, Quebec, Canada.
Immunology. 1988 May;64(1):175-80.
Twenty-three monoclonal antibodies (mAbs) against Giardia muris were obtained in two fusions using spleen cells of immunized mice. Similarly, the fusion of mesenteric lymph node (MLN) cells of an infected mouse yielded 15 mAbs. Three mAbs arising from spleen cells and two from MLN (all five were IgM) were able to kill trophozoites in the presence of guinea-pig complement (GPC). They can agglutinate trophozoites and also impair the movement of the flagella in the absence of GPC. I.p. treatment of mice with mAb 45C on Days -1, 1, 3 and 5 of infection significantly reduced the intestinal parasite burden. Indirect immunofluorescence assays on live and fixed trophozoites revealed that the cytotoxic mAbs were directed against antigens located on the periphery of the body, the sucking disc, the flagella and the ventro-lateral flange. In Western blots, our mAbs recognized major 36,200 and 30,300 MW glycoproteins located on the surface of the parasite.
利用免疫小鼠的脾细胞，通过两次融合获得了23种抗鼠贾第虫的单克隆抗体（mAb）。同样，用感染小鼠的肠系膜淋巴结（MLN）细胞进行融合，得到了15种单克隆抗体。来自脾细胞的3种单克隆抗体和来自MLN的2种单克隆抗体（这5种均为IgM）在豚鼠补体（GPC）存在的情况下能够杀死滋养体。在没有GPC的情况下，它们可以凝集滋养体并也会损害鞭毛的运动。在感染的第-1、1、3和5天，用单克隆抗体45C腹腔注射处理小鼠，可显著降低肠道寄生虫负荷。对活的和固定的滋养体进行间接免疫荧光分析表明，细胞毒性单克隆抗体针对的是位于虫体周边、吸盘、鞭毛和腹侧边缘的抗原。在蛋白质免疫印迹分析中，我们的单克隆抗体识别位于寄生虫表面的主要分子量为36200和30300的糖蛋白。
