Erlich J H, Anders R F, Roberts-Thomson I C, Schrader J W, Mitchell G F
Aust J Exp Biol Med Sci. 1983 Oct;61 ( Pt 5):599-615. doi: 10.1038/icb.1983.57.
After oral administration of cysts of the intestinal protozoan parasite, Giardia muris, young male C3H/He mice are chronically infected, whereas BALB/c mice demonstrate a rapidly resolving pattern of infection. Both strains of mice injected with trophozoites in adjuvant and challenged orally with cysts develop serum antibodies to numerous trophozoite proteins. A limited number of these protein antigens was differentially immunoprecipitated by sera from resistant BALB/c and susceptible C3H/He mice exposed to G. muris. 35S-methionine-labelled protein antigens better recognized by immune BALB/c sera included molecules of relative mobility (Mr) 82,000 and a series of proteins of Mr 25,000 to 32,000. Differential recognition extended to a subset of solubilized trophozoite antigens that bind to the lectin, wheat germ agglutinin (WGA), and that can be radio-iodinated. In particular, a complex of 4 acidic protein antigens of approximate Mr 32,000, and designated collectively as Gm32, was better recognized by immune BALB/c serum than C3H/He serum. Isolated WGA-binding antigens were not able to consistently vaccinate BALB/c mice against subsequent G. muris infection. Moreover, preliminary evidence has been obtained that lack of antibody responsiveness to Gm32 does not segregate strictly with susceptibility to chronic infection in (BALB/c X C3H/He)F2 mice. These data, plus the observation that drug-cured C3H/He mice are highly resistant to reinfection, has led to examination of whether mice differ in the capacity of the intestines to support inflammatory responses. Mast cell deficient Wf/Wf mice, unlike wild-type litter-mates, developed chronic giardiasis although no reconstitution of resistance has yet been achieved with inocula of bone marrow cells from +/+ mice. BALB/c mice injected with the antihistamine and antiserotonin drug, cyproheptadine, also showed prolonged infections with G. muris. The data suggest that analysis of specificity differences in immune responses of mice varying in susceptibility to intestinal parasites must be supplemented by examination of the capacity of the intestine to support induced immune responses.
口服肠道原生动物寄生虫微小贾第虫的包囊后，年轻雄性C3H/He小鼠会受到慢性感染，而BALB/c小鼠则表现出感染迅速消退的模式。用滋养体与佐剂混合注射并经口用包囊攻击的这两种品系小鼠，均产生了针对多种滋养体蛋白的血清抗体。抗性BALB/c小鼠和易感性C3H/He小鼠暴露于微小贾第虫后，其血清对有限数量的这些蛋白质抗原有不同的免疫沉淀作用。免疫BALB/c血清更易识别的经35S-甲硫氨酸标记的蛋白质抗原包括相对迁移率（Mr）为82,000的分子以及一系列Mr为25,000至32,000的蛋白质。这种差异识别还扩展到了与凝集素麦胚凝集素（WGA）结合且可进行放射性碘化的可溶性滋养体抗原亚群。特别是，一组约Mr为32,000的4种酸性蛋白质抗原复合物，统称为Gm32，免疫BALB/c血清比C3H/He血清更易识别。分离出的WGA结合抗原不能始终如一地使BALB/c小鼠对随后的微小贾第虫感染产生免疫。此外，已获得初步证据表明，在（BALB/c×C3H/He）F2小鼠中，对Gm32缺乏抗体反应性并不严格与对慢性感染的易感性相关。这些数据，再加上药物治愈的C3H/He小鼠对再次感染具有高度抗性这一观察结果，促使人们研究小鼠在肠道支持炎症反应的能力方面是否存在差异。与野生型同窝小鼠不同，肥大细胞缺陷的Wf/Wf小鼠患上了慢性贾第虫病，尽管用+/+小鼠的骨髓细胞接种尚未实现抗性重建。注射抗组胺和抗血清素药物赛庚啶的BALB/c小鼠，也表现出微小贾第虫感染持续时间延长。数据表明，对肠道寄生虫易感性不同的小鼠免疫反应特异性差异的分析，必须辅以对肠道支持诱导免疫反应能力的研究。
