LUNAM Université, ONIRIS, Université Nantes, UMR1014 Secalim, Nantes F-44307, France; INRA, Nantes F-44307, France; Ifremer, Laboratoire Science et Technologie de la Biomasse Marine, BP 21105, 44311 Nantes Cedex 3, France.
Ifremer, Laboratoire Science et Technologie de la Biomasse Marine, BP 21105, 44311 Nantes Cedex 3, France.
Food Microbiol. 2014 Jun;40:9-17. doi: 10.1016/j.fm.2013.11.018. Epub 2013 Dec 9.
The spoilage potential of isolates belonging to five bacterial groups/species (Shewanella baltica, Carnobacterium maltaromaticum, Aeromonas salmonicida, Vibrio sp., "other Gamma-Proteobacteria" [containing one strain of Pseudoalteromonas sp. and one strain of Psychrobacter sp.]) isolated from spoiled cooked and whole tropical shrimp stored under modified atmosphere packaging (MAP) was evaluated by inoculation into ionized cooked and peeled tropical shrimp followed by storage for 32 days at 8 °C. Microbial growth and sensory changes were monitored during the storage period. The major spoilage bacterial isolate groups were C. maltaromaticum and S. baltica. In order to characterize their spoilage potential further and to study the effect of their interactions, each of these two specific spoilage organisms (SSO) and one mixed-culture, C. maltaromaticum/S. baltica, were tested using a combination of complementary methods: molecular (PCR-TTGE), sensory, chemical, and conventional microbiological analyses. It was concluded that, in the mixed-culture-inoculated samples, both species groups imposed their spoilage characteristics.
从经改良气调包装（MAP）贮藏的变质熟制和整只热带虾中分离出的 5 个细菌种群/物种（希瓦氏菌属、乳杆菌属、鲑鱼气单胞菌、弧菌属、“其他γ-变形菌”[含一株假交替单胞菌和一株嗜冷杆菌]）的腐败潜能，通过接种离子化熟制和去皮热带虾，并在 8°C 下贮藏 32 天进行评估。在贮藏期间监测微生物生长和感官变化。主要腐败细菌分离种群为乳杆菌属和希瓦氏菌属。为了进一步表征它们的腐败潜力，并研究它们相互作用的影响，使用互补方法（PCR-TTGE、感官、化学和常规微生物分析）对这两种特定腐败生物（SSO）和一种混合培养物（乳杆菌属/希瓦氏菌属）进行了测试。结论是，在混合培养物接种的样品中，两个种群都表现出了腐败特征。
