用于小分子染料的RNA适配体的体外筛选。

In vitro selection of RNA aptamers for a small-molecule dye.

作者信息

Murata Asako, Sato Shin-ichi

机构信息

The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Japan.

出版信息

Methods Mol Biol. 2014;1111:17-28. doi: 10.1007/978-1-62703-755-6_2.

DOI:10.1007/978-1-62703-755-6_2

PMID:24549609

Abstract

Artificial riboswitches can be generated by functional coupling of an RNA aptamer for synthetic small molecule to an expression platform. RNA aptamers that can bind strongly and selectively to their target are feasible to be used for obtaining more potent artificial riboswitches. In this chapter, we describe tips and notes for in vitro selection of RNA aptamers targeting synthetic small molecules.

摘要

人工核糖开关可通过将用于合成小分子的RNA适体与一个表达平台进行功能偶联来产生。能够与其靶标强烈且选择性结合的RNA适体可用于获得更有效的人工核糖开关。在本章中，我们描述了针对合成小分子的RNA适体体外筛选的提示和注意事项。