Awad Wageha A, Molnár Andor, Aschenbach Jörg R, Ghareeb Khaled, Khayal Basel, Hess Claudia, Liebhart Dieter, Dublecz Károly, Hess Michael
Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria
Department of Animal Science and Animal Husbandry, Georgikon Faculty, University of Pannonia, Keszthely, Hungary.
Innate Immun. 2015 Feb;21(2):151-60. doi: 10.1177/1753425914521648. Epub 2014 Feb 19.
Asymptomatic carriage of Campylobacter jejuni is highly prevalent in chicken flocks. Thus, we investigated whether chronic Campylobacter carriage affects chicken intestinal functions despite the absence of clinical symptoms. An experiment was carried out in which commercial chickens were orally infected with C. jejuni (1 × 10(8) CFU/bird) at 14 days of life. Changes in ion transport and barrier function were assessed by short-circuit current (I(sc)) and transepithelial ion conductance (G(t)) in Ussing chambers. G(t) increased in cecum and colon of Campylobacter-infected chicken 7 d post-infection (DPI), whereas G t initially decreased in the jejunum at 7 DPI and increased thereafter at 14 DPI. The net charge transfer across the epithelium was reduced or tended to be reduced in all segments, as evidenced by a decreased I sc. Furthermore, the infection induced intestinal histomorphological changes, most prominently including a decrease in villus height, crypt depth and villus surface area in the jejunum at 7 DPI. Furthermore, body mass gain was decreased by Campylobacter carriage. This study demonstrates, for the first time, changes in the intestinal barrier function in Campylobacter-infected chickens and these changes were associated with a decrease in growth performance in otherwise healthy-appearing birds.
空肠弯曲菌的无症状携带在鸡群中非常普遍。因此，我们研究了尽管没有临床症状，但空肠弯曲菌的慢性携带是否会影响鸡的肠道功能。进行了一项实验，在14日龄时给商用鸡口服感染空肠弯曲菌（1×10⁸CFU/只）。通过Ussing小室中的短路电流（Isc）和跨上皮离子电导（Gt）评估离子转运和屏障功能的变化。感染空肠弯曲菌的鸡在感染后7天（DPI），盲肠和结肠中的Gt增加，而空肠中的Gt在7 DPI时最初下降，随后在14 DPI时增加。如Isc降低所示，所有肠段上皮的净电荷转移减少或趋于减少。此外，感染引起肠道组织形态学变化，最明显的是在7 DPI时空肠绒毛高度、隐窝深度和绒毛表面积减少。此外，空肠弯曲菌携带导致体重增加减少。这项研究首次证明了感染空肠弯曲菌的鸡肠道屏障功能的变化，并且这些变化与外观健康的鸡的生长性能下降有关。
