Łasica Anna, Godlewska Renata, Gubernator Jerzy, Jakubiak-Augustyn Anna, Majewski Paweł, Wyszyńska Agnieszka
Department of Bacterial Genetics, Institute of Microbiology, Warszawa, Poland.
Department of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, 50-300 Wrocław, Poland.
J Vet Res. 2024 Nov 6;68(4):487-496. doi: 10.2478/jvetres-2024-0062. eCollection 2024 Dec.
are the most common cause of food poisoning, which manifests itself in diarrhoea of varying severity. Additionally, because of the increasing number of people with immune deficiencies, more frequent serious complications of infections are being observed. The main source of infection is the consumption of contaminated poultry meat, which is a consequence of the insufficiency of current hygiene and biosecurity to control or eliminate it from the poultry food chain.
Two hybrid proteins, presenting selected epitopes of the antigens CjaD and EF-Tu, were developed based on the highly immunogenic proteins CjaA and CjaC. Four groups of chickens were vaccinated with different preparations (a mixture of both hybrid proteins encapsulated in anionic or neutral liposomes) and different doses (a single dose given on the day of hatching or two doses given on days 1 and 14 of life). The number of was assessed in the intestinal contents of vaccinated birds.
No statistically significant differences in colonisation levels were observed between chickens immunised with neutral liposomes containing hybrid proteins and their non-immunised counterparts, regardless of dosage regimen.
Although immunisation of chickens did not produce the expected results, the approach used has great potential, which is worth further investigation and development.
是食物中毒最常见的原因，表现为不同严重程度的腹泻。此外，由于免疫缺陷人群数量不断增加，感染引发的严重并发症日益常见。感染的主要来源是食用受污染的禽肉，这是当前卫生和生物安全措施不足以控制或从禽肉食物链中消除污染的结果。
基于高免疫原性蛋白CjaA和CjaC，开发了两种呈现抗原CjaD和EF-Tu选定表位的杂交蛋白。四组鸡用不同制剂（封装在阴离子或中性脂质体中的两种杂交蛋白混合物）和不同剂量（孵化当天单剂量给药或出生后第1天和第14天两剂量给药）进行免疫接种。对接种鸡肠道内容物中的数量进行评估。
无论给药方案如何，用含杂交蛋白的中性脂质体免疫的鸡与其未免疫的对应鸡之间，在定殖水平上未观察到统计学显著差异。
尽管对鸡的免疫接种未产生预期结果，但所采用的方法具有很大潜力，值得进一步研究和开发。
