Department of Food Technology, XaRTA-UTPV, Escola Tècnica Superior d'Enginyeria Agrària, Universitat de Lleida, Avda/Alcalde Rovira Roure 191, 25198 Lleida, Spain.
Food Funct. 2014 Apr;5(4):740-7. doi: 10.1039/c3fo60446b.
The aim of this work was to examine whether bioactives in thyme could enhance the antioxidant capacity of phenolics in virgin olive oil and their bioavailability in Wistar rats. After acute oral administration of extracts from olive cake (OE), thyme (TE) or their combination (OTE), blood samples were collected from 0 to 360 min. Plasma antioxidant status was analyzed by DPPH and FRAP in plasma and by SOD, CAT and GPx activities in erythrocytes. Plasma pharmacokinetics of the main metabolites of bioactives in olive oil and thyme were characterized. Plasma non-enzymatic antioxidant capacity was significantly modulated by OE, TE, and OTE in a time-, assay, and extract-dependent manner. OE, TE, and OTE all significantly decreased superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity and catalase (CAT) activity was increased. Pharmacokinetic results showed that plasma concentration (Cmax) of the main olive phenolic metabolites in rats fed with OTE were similar to those of OE. These results indicate that an enhanced bioavailability of olive phenolic compounds could occur in the presence of thyme, although any synergistic effect was observed in the antioxidant status when both phenolic extracts were administered. Antioxidant protection by phenolics from olive and thyme against oxidative stress occurs primarily through a direct antioxidant effect and may be related to the phenolic plasmatic metabolites.
本研究旨在探讨百里香中的生物活性物质是否能增强橄榄油中原生多酚的抗氧化能力及其在 Wistar 大鼠体内的生物利用度。在急性口服橄榄油饼（OE）、百里香（TE）或它们的混合物（OTE）提取物后，从 0 到 360 分钟采集血液样本。通过 DPPH 和 FRAP 分析血浆抗氧化状态，通过 SOD、CAT 和 GPx 活性分析红细胞抗氧化状态。对橄榄油和百里香中生物活性物质的主要代谢物在血浆中的药代动力学进行了表征。OE、TE 和 OTE 以时间、测定和提取物依赖的方式显著调节了血浆非酶抗氧化能力。OE、TE 和 OTE 均显著降低了超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的活性，同时增加了过氧化氢酶（CAT）的活性。药代动力学结果表明，给予 OTE 的大鼠血浆中橄榄油主要酚类代谢物的浓度（Cmax）与 OE 相似。这些结果表明，在百里香存在的情况下，橄榄油酚类化合物的生物利用度可能会增强，尽管当两种酚类提取物同时给药时，抗氧化状态没有观察到协同作用。橄榄油和百里香中的酚类化合物通过直接抗氧化作用对氧化应激产生的抗氧化保护作用可能与酚类血浆代谢物有关。
