Zhu Yu, Schreiter Sven, Tanaka Elly M
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Methods Mol Biol. 2016;1307:345-55. doi: 10.1007/7651_2013_56.
Successful applications of pluripotent stem cells to cell-based therapies will rely on rapid and efficient methods to differentiate cells toward the target cell type. While methods have been developed for the generation of some medically relevant cell types including retinal pigment epithelium (RPE) cells, such protocols are lengthy and result in a heterogeneous cell mixture of RPE and non-RPE cells, requiring manual subselection and expansion. Such considerations have significant limiting impact of therapeutic applicability. Here we describe the accelerated three-dimensional neuroepithelial cyst culture of human embryonic stem cells (hESCs) and its utility to achieve quantitative production of RPE cell sheet with no manual selection in 30 days.
多能干细胞在基于细胞的治疗中的成功应用将依赖于快速有效的方法，使细胞向目标细胞类型分化。虽然已经开发出了一些生成包括视网膜色素上皮（RPE）细胞在内的某些医学相关细胞类型的方法，但这些方案耗时较长，并且会产生RPE细胞和非RPE细胞的异质细胞混合物，需要手动进行亚选择和扩增。这些因素对治疗的适用性有很大的限制影响。在此，我们描述了人类胚胎干细胞（hESCs）的加速三维神经上皮囊肿培养及其在30天内无需手动选择即可定量生产RPE细胞片的效用。
