Pyne Michael T, Law Christian, Hillyard David R, Schlaberg Robert
ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
J Clin Microbiol. 2014 May;52(5):1720-3. doi: 10.1128/JCM.03308-13. Epub 2014 Feb 19.
The accurate detection and typing of high-risk human papillomavirus (HPV) are critical for cervical cancer screening. The Hybrid Capture 2 (hc2) and cobas HPV tests showed high agreement for cervical samples (94.4%, κ=0.72, n=693) and moderate agreement for vaginal samples (κ=0.62, n=108). The HPV16 and HPV18 results were highly consistent between the cobas and Linear Array tests (κ≥0.96, n=197). Three hc2-negative vaginal samples were repeatedly invalid by the cobas test due to β-globin control failures, highlighting amplification control benefits. No cross-contamination was detected in a challenge experiment.
高危型人乳头瘤病毒（HPV）的准确检测和分型对于宫颈癌筛查至关重要。杂交捕获2代（hc2）和cobas HPV检测对宫颈样本显示出高度一致性（94.4%，κ=0.72，n=693），对阴道样本显示出中度一致性（κ=0.62，n=108）。cobas检测和线性阵列检测之间的HPV16和HPV18结果高度一致（κ≥0.96，n=197）。由于β-珠蛋白对照失败，3份hc2检测阴性的阴道样本经cobas检测反复无效，突出了扩增对照的益处。在一项挑战实验中未检测到交叉污染。
