Department of Botany, University of Delhi, Delhi, 110007 India.
Department of Botany, Shivaji University, Kolhapur, Maharashtra India.
Physiol Mol Biol Plants. 2014 Jan;20(1):103-14. doi: 10.1007/s12298-013-0206-2. Epub 2013 Dec 18.
Genetic diversity and relationship among three genera namely Drimia, Dipcadi and Ledebouria of Hyacinthaceae in India was studied using RAPD and SRAP markers. Twenty one RAPD primers and nine SRAP were used for analyzing 41 accessions. RAPD gave an average 12.6 markers per primer, while SRAP generated 10.1 markers per primer pair. The family emerged very diverged with high polymorphism. The study resolved the three genera into monophyletic groups corresponding to three subfamilies; Urginoideae, Hyacinthoideae and Ornithogaloideae. Drimia wightii emerged a very distinct species and species specific markers were obtained with both marker systems. AMOVA analysis also revealed the genera to be quite well diverged. The two markers showed high correlation (r = 0.932) in Mantel matrix crresspondance test. The combined data also showed a very good correlation with the respective markers individually.
采用 RAPD 和 SRAP 标记对石蒜科的 3 个属（Drimia、Dipcadi 和 Ledebouria）在印度的遗传多样性和关系进行了研究。使用 21 个 RAPD 引物和 9 个 SRAP 引物对 41 个材料进行了分析。RAPD 平均每个引物产生 12.6 个标记，而 SRAP 每个引物对产生 10.1 个标记。该科的多态性很高，呈现出高度分化。研究结果将这 3 个属分为 3 个亚科，即 Urginoideae、Hyacinthoideae 和 Ornithogaloideae，分别对应单系群。Drimia wightii 是一个非常独特的物种，两种标记系统都获得了该物种特有的标记。AMOVA 分析也表明这些属分化得很好。Mantel 矩阵对应分析表明，两种标记的相关性很高（r=0.932）。合并数据与各自的标记也具有很好的相关性。
