硫酸葡聚糖降低家族性高胆固醇血症患者的低密度脂蛋白水平。

Reduction of low-density lipoproteins with dextran sulfate in patients with familial hypercholesterolemia.

作者信息

Antwiler G D, Dau P C, Lobdell D D

机构信息

COBE Laboratories, Inc., Lakewood, Colorado 80215.

出版信息

J Clin Apher. 1988;4(1):18-29. doi: 10.1002/jca.2920040106.

DOI:10.1002/jca.2920040106

PMID:2455708

Abstract

A novel on-line system for the selective precipitation of low-density lipoprotein (LDL) using dextran sulfate has been devised and tested in four patients with heterozygous familial hypercholesterolemia (type II). The mean pretreatment serum cholesterol was 410 mg/dl. Plasma was generated by membrane filtration and LDL and VLDL (very-low-density lipoprotein) were completely precipitated with 10-35 mg% dextran sulfate (Mr 5,000) in the presence of 55 mM Ca2+. The precipitate was removed by filtration and the excess Ca2+ by dialysis. For 41 procedures the mean reduction of plasma solutes was LDL + VLDL 65%, HDL 23%, fibrinogen 19%, albumin 15%, IgG 20%, IgA 19%, IgM 24%. We conclude that dextran sulfate precipitation is an effective method for selective on-line removal of LDL from plasma.

摘要

一种使用硫酸葡聚糖选择性沉淀低密度脂蛋白（LDL）的新型在线系统已被设计出来，并在4名杂合子家族性高胆固醇血症（II型）患者身上进行了测试。治疗前血清胆固醇平均为410mg/dl。通过膜过滤产生血浆，在55mM Ca2+存在下，用10 - 35mg%硫酸葡聚糖（分子量5000）使LDL和极低密度脂蛋白（VLDL）完全沉淀。通过过滤除去沉淀物，通过透析除去过量的Ca2+。在41次操作中，血浆溶质的平均减少量为：LDL + VLDL 65%，高密度脂蛋白（HDL）23%，纤维蛋白原19%，白蛋白15%，免疫球蛋白G（IgG）20%，免疫球蛋白A（IgA）19%，免疫球蛋白M（IgM）24%。我们得出结论，硫酸葡聚糖沉淀是从血浆中选择性在线去除LDL的有效方法。