Sati Jyoti, Sah Seema, Pandey Himani, Ali Shahnawaz, Sahoo Prabhati Kumari, Pande Veena, Baratz Ashoktaru
Department of Biotechnology, Kumaun University, Nainital-263 136, India.
Molecular Genetics Laboratory, Directorate of Coldwater Fisheries Research, Bhimtal-263 136, India.
J Environ Biol. 2013 Sep;34(5):933-9.
This study examined the phylogenetic relationship and identification of five Mahseer species (Tor putitoro, Tor tor, Tor khudree, Tor chelynoides and Neolissochilus hexogonolopis) using partial sequencing of a Cytochrome Oxidase I (COl) DNA barcodes. The sequence analysis data showed that 134 (21.61%) sites out of 628 sites were variable without insertion or deletion. Rate of transition (70.5%) were higher than transversion (29.41%). There was a high inter-species divergence (range 4.1% to 12.2%) in Mahseer species as compared to intra-specific sequence divergence (1.7% for T. putitora, 1.2% for T tor, 1.4% for T. khudree, 3.0% for T chelynoides, 0.26 % for N. hexagonolopis). The phylogenetic tree, constructed by maximum parsimony, maximum likelihood and unweighted pair group average methods revealed similar results suggesting that T. putitoro, T. tor and T. khudree had a close relationship to each other while maximum divergence was observed in T. chelynoides, which was also confirmed by the genetic distance data. The results indicate that COl sequencing or bar-coding is useful in unravelling phylogenetic relationship and identification of Mahseer species.
本研究利用细胞色素氧化酶I（COl）DNA条形码的部分序列，研究了五种马西亚鱼（托氏野鲮、杰氏野鲮、库氏野鲮、似野鲮和六须新光唇鱼）的系统发育关系并进行了鉴定。序列分析数据显示，在628个位点中，有134个（21.61%）位点可变，无插入或缺失。转换率（70.5%）高于颠换率（29.41%）。与种内序列差异相比，马西亚鱼种间差异较大（范围为4.1%至12.2%）（托氏野鲮为1.7%，杰氏野鲮为1.2%，库氏野鲮为1.4%，似野鲮为3.0%，六须新光唇鱼为0.26%）。通过最大简约法、最大似然法和非加权配对组平均法构建的系统发育树显示了相似的结果，表明托氏野鲮、杰氏野鲮和库氏野鲮之间关系密切，而似野鲮的差异最大，这也得到了遗传距离数据的证实。结果表明，COl测序或条形码技术有助于阐明马西亚鱼的系统发育关系并进行物种鉴定。
