独活寄生汤水提取物对软骨细胞G1期调节因子mRNA表达的影响
[Effect of water extracts from duhuo jisheng decoction on expression of chondrocyte G1 phase regulator mRNA].
作者信息
Chen Jia-Shou, Li Xi-Hai, Li Hui-Ting, Weng Xia-Ping, Xu Hui-Feng, Ye Hong-Zhi, Liu Xian-Xiang
机构信息
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
Research Institute of Integrated Traditional Chinese and Western Medicines, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2013 Nov;38(22):3949-52.
OBJECTIVE
To investigate the effect of water extracts from Duhuo Jisheng decoction on chondrocyte G1 phase.
METHODS
Chondrocytes were collected from four-week-old SD rats to establish the chondrocyte in vitro culture system. The third generation of chondrocytes was intervened. MTT method was used to measure the effect of water extracts from different concentrations of Duhuo Jisheng decoction on chondrocyte activity. The expressions of Chondrocyte Cyclin D1, CDK4, CDK6 and P21 mRNA in the blank group and low, middle and high-dose groups (100, 200, 400 mg x L(-1)) were detected by RT-PCR method.
RESULT
The MTT assay showed that the chondrocyte activity significantly increased within specific drug concentrations (50-800 mg x L(-1)) (P < 0.01); After the intervention for 24 h, the expressions of CyclinD1, CDK4 and CDK6 mRNA in all dose groups notably increased (P < 0.05), with the maximum expressions at the concentration of 200 mg x L(-1); The expression of P21 mRNA decreased, particularly at the concentration of 200 mg x L(-1) (P < 0.01).
CONCLUSION
Water extracts from Duhuo Jisheng decoction can promote chondrocyte proliferation by effecting the expression of chondrocyte G1 phase regulator mRNA.
目的
探讨独活寄生汤水提物对软骨细胞G1期的影响。
方法
取四周龄SD大鼠软骨细胞，建立软骨细胞体外培养体系。对第三代软骨细胞进行干预。采用MTT法检测不同浓度独活寄生汤水提物对软骨细胞活性的影响。采用RT-PCR法检测空白组及低、中、高剂量组（100、200、400 mg·L⁻¹）软骨细胞周期蛋白D1（Cyclin D1）、细胞周期蛋白依赖性激酶4（CDK4）、细胞周期蛋白依赖性激酶6（CDK6）及P21 mRNA的表达。
结果
MTT法检测结果显示，在特定药物浓度（50 - 800 mg·L⁻¹）范围内软骨细胞活性显著升高（P < 0.01）；干预24 h后，各剂量组CyclinD1、CDK4及CDK6 mRNA表达均显著升高（P < 0.05），在200 mg·L⁻¹浓度时表达量最高；P21 mRNA表达降低，在200 mg·L⁻¹浓度时降低尤为明显（P < 0.01）。
结论
独活寄生汤水提物可通过影响软骨细胞G1期调节因子mRNA的表达促进软骨细胞增殖。
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