Department of Traditional Chinese Medicine, ZhuJiang Hospital, Southern Medical University, Guangzhou 510280, China; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
Int Immunopharmacol. 2014 Apr;19(2):358-64. doi: 10.1016/j.intimp.2014.02.007. Epub 2014 Feb 21.
Ligustilide, the major component of Angelica sinensis, is also thought to be the most potent bioactive constituent of this plant. Ligustilide has been reported to markedly protect neural tissue against apoptosis. However, little is known regarding ligustilide's anti-apoptosis effect in muscle tissue. The aim of the study was to investigate the anti-apoptosis effects of ligustilide on TNF-α-induced C2C12 cells during differentiation. It was revealed that ligustilide at various concentrations significantly prevented the apoptosis of C2C12 cells incubated in TNF-α as assessed by apoptosis index and DNA fragmentation. Moreover, ligustilide-treated groups exhibited a significant increase in the bcl-2/bax ratio, pro-caspase 3 and pro-caspase 8 compared with the TNF-α-control group in a dose-dependent manner. Meanwhile, ligustilide-treated groups presented a significantly increased level of phosphorylated Akt and suppressed expression of the myogenin protein. Therefore, the findings derived suggested that ligustilide protected C2C12 cells from TNF-α-induced apoptosis during differentiation by reducing apoptosis and inducing cell proliferation.
藁本内酯是当归的主要成分，也是该植物中最有效的生物活性成分。藁本内酯已被报道能显著保护神经组织免受细胞凋亡。然而，关于藁本内酯在肌肉组织中的抗细胞凋亡作用知之甚少。本研究旨在探讨藁本内酯对 TNF-α诱导的分化过程中 C2C12 细胞的抗凋亡作用。结果表明，通过凋亡指数和 DNA 片段化检测，不同浓度的藁本内酯能显著防止 TNF-α孵育的 C2C12 细胞凋亡。此外，与 TNF-α 对照组相比，藁本内酯处理组的 bcl-2/bax 比值、原半胱氨酸蛋白酶 3 和原半胱氨酸蛋白酶 8 呈剂量依赖性增加。同时，藁本内酯处理组呈现出磷酸化 Akt 水平的显著增加，并抑制肌球蛋白蛋白的表达。因此，研究结果表明，藁本内酯通过减少细胞凋亡和诱导细胞增殖来保护 C2C12 细胞免受 TNF-α诱导的分化过程中的细胞凋亡。
