Allen M H, Tidman M J, MacDonald D M
Laboratory of Applied Dermatopathology, United Medical School of Guy's Hospital, London, U.K.
Br J Dermatol. 1988 May;118(5):645-50. doi: 10.1111/j.1365-2133.1988.tb02565.x.
Immunogold-silver staining (IGSS) was evaluated for the localization of cutaneous antigens in frozen and paraffin-embedded tissue, employing antibodies to a variety of intracellular, cell-surface and extracellular epitopes in an indirect immunogold reaction, followed by silver enhancement. The principal advantages of IGSS are the avoidance of toxic reagents and the production of a silver precipitate that is permanent, clearly visible and of sufficient contrast with the reaction products of alternative immunolocalizing techniques to be of value in double-labelling procedures. It seems to localize antigenic determinants well, but does not appear to be particularly sensitive, especially for the demonstration of antibodies to extracellular constituents. Limitations of IGSS include the propensity to non-specific staining, and the need to vary the duration of the development stage during silver enhancement.
免疫金银染色法（IGSS）用于冷冻组织和石蜡包埋组织中皮肤抗原的定位，在间接免疫金反应中使用针对多种细胞内、细胞表面和细胞外表位的抗体，随后进行银增强。IGSS的主要优点是避免使用有毒试剂，并且产生的银沉淀是永久性的，清晰可见，与其他免疫定位技术的反应产物具有足够的对比度，在双重标记程序中具有价值。它似乎能很好地定位抗原决定簇，但似乎不是特别敏感，尤其是对于细胞外成分抗体的检测。IGSS的局限性包括容易出现非特异性染色，以及在银增强过程中需要改变显影阶段的持续时间。
