Liu Cunbao, Yang Xu, Yao Yufeng, Huang Weiwei, Sun Wenjia, Ma Yanbing
Department of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Engineering Research Center of Vaccine Research & Development on Severe Infectious Diseases, #935 Jiaoling Road, Kunming, 650118, Yunnan, People's Republic of China,
Biotechnol Lett. 2014 May;36(5):937-45. doi: 10.1007/s10529-014-1455-z. Epub 2014 Feb 22.
Two versions of an optimized gene that encodes human papilloma virus type 16 major protein L1 were designed according to the codon usage frequency of Pichia pastoris. Y16 was highly expressed in both P. pastoris and Hansenula polymorpha. M16 expression was as efficient as that of Y16 in P. pastoris, but merely detectable in H. polymorpha even though transcription levels of M16 and Y16 were similar. H. polymorpha had a unique codon usage frequency that contains many more rare codons than Saccharomyces cerevisiae or P. pastoris. These findings indicate that even codon-optimized genes that are expressed well in S. cerevisiae and P. pastoris may be inefficiently expressed in H. polymorpha; thus rare codons must be avoided when universal optimized gene versions are designed to facilitate expression in a variety of yeast expression systems, especially H. polymorpha is involved.
根据毕赤酵母的密码子使用频率，设计了编码人乳头瘤病毒16型主要蛋白L1的优化基因的两个版本。Y16在毕赤酵母和多形汉逊酵母中均高表达。M16在毕赤酵母中的表达效率与Y16相当，但在多形汉逊酵母中即使M16和Y16的转录水平相似也仅能检测到。多形汉逊酵母具有独特的密码子使用频率，其稀有密码子比酿酒酵母或毕赤酵母多得多。这些发现表明，即使是在酿酒酵母和毕赤酵母中高效表达的密码子优化基因，在多形汉逊酵母中也可能表达效率低下；因此，在设计通用优化基因版本以促进在各种酵母表达系统中表达时，尤其是涉及多形汉逊酵母时，必须避免稀有密码子。
