Sciscione Fabiola, Pucci Carlotta, La Mesa Camillo
Department of Chemistry, Cannizzaro Building, La Sapienza University , P.le A. Moro 5, I-00185 Rome, Italy.
Langmuir. 2014 Mar 18;30(10):2810-9. doi: 10.1021/la500199w. Epub 2014 Mar 6.
Catanionic vesicles were prepared by mixing nonstoichiometric amounts of sodium bis(2-ethylhexyl) sulfosuccinate and dioctyldimethylammonium bromide in water. Depending on the concentration and mole ratios between the surfactants, catanionic vesicular aggregates are formed. They have either negative or positive charges in excess and are endowed with significant thermodynamic and kinetic stability. Vesicle characterization was performed by dynamic light scattering and electrophoretic mobility. It was inferred that vesicle size scales in inverse proportion with its surface charge density and diverges as the latter quantity approaches zero and/or the mole ratio equals unity. Therefore, both variables are controlled by the anionic/cationic mole ratio. Small-angle X-ray scattering, in addition, indicates that vesicles are unilamellar. Selected anionic vesicular systems were reacted with poly-L-lysine hydrobromide or lysozyme. Polymer binding continues until complete neutralization of the negatively charged sites on the vesicles surface is attained, as inferred by electrophoretic mobility. Lipoplexes are formed as a result of significant electrostatic interactions between cationic polyelectrolytes and negatively charged vesicles.
通过在水中混合非化学计量的双（2-乙基己基）磺基琥珀酸钠和二辛基二甲基溴化铵来制备阴阳离子囊泡。根据表面活性剂之间的浓度和摩尔比，形成阴阳离子囊泡聚集体。它们带有过量的负电荷或正电荷，并具有显著的热力学和动力学稳定性。通过动态光散射和电泳迁移率对囊泡进行表征。据推断，囊泡大小与其表面电荷密度成反比，并且当后者接近零和/或摩尔比等于1时会发散。因此，这两个变量都由阴离子/阳离子摩尔比控制。此外，小角X射线散射表明囊泡是单层的。选择的阴离子囊泡系统与聚-L-赖氨酸氢溴酸盐或溶菌酶反应。如通过电泳迁移率推断的那样，聚合物结合持续进行，直到囊泡表面带负电的位点完全被中和。由于阳离子聚电解质与带负电的囊泡之间存在显著的静电相互作用，从而形成了脂质体。
