BMC Genomics. 2013;14 Suppl 8(Suppl 8):S6. doi: 10.1186/1471-2164-14-S8-S6. Epub 2013 Dec 9.
The gene Polymorphic derived intron-containing, known as Pldi, is a long non-coding RNA (lncRNA) first discovered in mouse. Although parts of its sequence were reported to be conserved in rat and human, it can only be expressed in mouse testis with a mouse-specific transcription start site. The consensus sequence of Pldi is also part of an antisense transcript AK158810 expressed in a wide range of mouse tissues.
We focused on sequence origin of Pldi and Ak158810. We demonstrated that their sequence was originated from an inter-genic region and is only presented in mammalians. Transposable events and chromosome rearrangements were involved in the evolution of ancestral sequence. Moreover, we discovered high conservation in part of this region was correlated with chromosome rearrangements, CpG demethylation and transcriptional factor binding motif. These results demonstrated that multiple factors contributed to the sequence origin of Pldi.
We comprehensively analyzed the sequence origin of Pldi-Ak158810 loci. We provided various factors, including rearrangement, transposable elements, contributed to the formation of the sequence.
多态性衍生内含子基因，称为 Pldi，是第一个在老鼠中发现的长非编码 RNA（lncRNA）。尽管其部分序列在大鼠和人类中被报道具有保守性，但它只能在具有特定转录起始位点的小鼠睾丸中表达。Pldi 的保守序列也是在广泛的小鼠组织中表达的反义转录本 AK158810 的一部分。
我们专注于 Pldi 和 Ak158810 的序列起源。我们证明它们的序列来源于基因间区，仅存在于哺乳动物中。转座事件和染色体重排参与了祖先序列的进化。此外，我们发现该区域的部分高度保守性与染色体重排、CpG 去甲基化和转录因子结合基序相关。这些结果表明，多种因素导致了 Pldi 的序列起源。
我们全面分析了 Pldi-Ak158810 基因座的序列起源。我们提供了多种因素，包括重排、转座元件，它们有助于序列的形成。
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