Wang Y P, Wei J Y, Yang J J, Gao W N, Wu J Q, Guo C J
Institute of Health and Environmental Medicine, Tianjin, P. R. China.
Physiol Res. 2014;63(3):341-50. doi: 10.33549/physiolres.932670. Epub 2014 Feb 24.
This study investigated the effects of riboflavin on energy metabolism in hypoxic mice. Kunming mice were fed diets containing riboflavin at doses of 6, 12, 24 and 48 mg/kg, respectively for 2 weeks before exposure to a simulated altitude of 6000 m for 8 h. Changes of riboflavin status and energy metabolism were assessed biochemically. Simultaneously, a (1)H nuclear magnetic resonance (NMR) based metabolomic technique was used to track the changes of plasma metabolic profiling. It was found that the content of hepatic riboflavin was decreased and erythrocyte glutathione activation coefficient was elevated significantly under hypoxic condition. Meanwhile, increased plasma pyruvate, lactate, beta-hydroxybutyrate and urea, as well as decreased plasma carnitine were observed. Riboflavin supplementation improved riboflavin status remarkably in hypoxic mice and decreased plasma levels of pyruvate, free fatty acids and beta-hydroxybutyrate significantly. Plasma carnitine was increased in response to riboflavin supplementation. Results obtained from (1)H NMR analysis were basically in line with the data from biochemical assays and remarkable changes in plasma taurine, choline and some other metabolites were also indicated. It was concluded that riboflavin requirement was increased under acute hypoxic condition and riboflavin supplementation was effective in improving energy metabolism in hypoxic mice.
本研究调查了核黄素对缺氧小鼠能量代谢的影响。将昆明小鼠分别喂食含6、12、24和48 mg/kg剂量核黄素的日粮，持续2周，然后暴露于模拟海拔6000米的环境中8小时。通过生化方法评估核黄素状态和能量代谢的变化。同时，使用基于氢核磁共振（NMR）的代谢组学技术追踪血浆代谢谱的变化。结果发现，在缺氧条件下，肝脏核黄素含量降低，红细胞谷胱甘肽活化系数显著升高。同时，观察到血浆丙酮酸、乳酸、β-羟基丁酸和尿素增加，以及血浆肉碱减少。补充核黄素可显著改善缺氧小鼠的核黄素状态，并显著降低血浆丙酮酸、游离脂肪酸和β-羟基丁酸水平。补充核黄素后血浆肉碱增加。氢核磁共振分析得到的结果与生化分析数据基本一致，并且还表明血浆牛磺酸、胆碱和其他一些代谢物有显著变化。得出的结论是，急性缺氧条件下核黄素需求量增加，补充核黄素对改善缺氧小鼠的能量代谢有效。
