Kumar S, Miller L H, Quakyi I A, Keister D B, Houghten R A, Maloy W L, Moss B, Berzofsky J A, Good M F
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Nature. 1988 Jul 21;334(6179):258-60. doi: 10.1038/334258a0.
Malaria is initiated by the inoculation of a susceptible host with sporozoites from an infected mosquito. The sporozoites enter hepatocytes and develop for a period as exoerythrocyte or hepatic stage parasites. Vaccination with irradiated sporozoites can provide protective immunity and a recent study shows that this can also be conferred by immunization with a recombinant salmonella expressing only the circumsporozoite protein that normally covers the sporozoites. Protection against infection is likely to be mediated by cytotoxic CD8+ cells, as depletion of CD8+ T cells in a sporozoite-immunized animal can completely abrogate immunity. Here we demonstrate directly the existence of CD8+ cytotoxic T lymphocytes (CTL) that recognize the circumsporozoite protein. B10.BR mice immunized with sporozoites or with recombinant vaccinia virus expressing the CS protein of Plasmodium falciparum contain CTL that specifically kill L cell fibroblasts transfected with the gene encoding the same CS protein. The peptide epitope from the CS protein that is recognized by CTL from this strain of mice is from a variant region of the protein.
疟疾是由受感染蚊子的子孢子接种易感宿主引发的。子孢子进入肝细胞，并作为红细胞外期或肝期寄生虫发育一段时间。用经辐射的子孢子进行疫苗接种可提供保护性免疫，最近一项研究表明，仅表达通常覆盖子孢子的环子孢子蛋白的重组沙门氏菌免疫也能赋予这种免疫。针对感染的保护作用可能由细胞毒性CD8 + 细胞介导，因为在经子孢子免疫的动物中耗尽CD8 + T细胞可完全消除免疫力。在这里，我们直接证明了识别环子孢子蛋白的CD8 + 细胞毒性T淋巴细胞（CTL）的存在。用子孢子或表达恶性疟原虫CS蛋白的重组痘苗病毒免疫的B10.BR小鼠含有CTL，这些CTL能特异性杀死用编码相同CS蛋白的基因转染的L细胞成纤维细胞。来自该品系小鼠的CTL识别的CS蛋白肽表位来自该蛋白的一个变异区域。
