Gross R A, Macdonald R L
Department of Neurology, University of Michigan Medical Center, Ann Arbor 48104-1687.
Neurosci Lett. 1988 May 16;88(1):50-6. doi: 10.1016/0304-3940(88)90314-x.
Voltage-clamp techniques were used to study the effect of forskolin, 8-Br-cyclic AMP, and phorbol 12,13-dibutyrate (PDBu) on the 3 voltage-dependent calcium current components of mouse dorsal root ganglion neurons in culture. Forskolin and 8-Br-cyclic AMP selectively reduced the transient high-threshold (N-type) calcium current component, an effect also produced by PDBu, a C kinase activator. Pretreatment of cultures with pertussis toxin prevented the reduction of calcium current by PDBu, but was without effect on the reductions produced by forskolin or 8-Br-cyclic AMP. These results support the contention that activation of both A and C kinases selectively affect calcium currents in vertebrate neurons by reducing the N-type calcium current component. While the activation of the C kinase required a G protein to exert its effect, the activation of A kinase did not.
采用电压钳技术研究了毛喉素、8-溴环磷酸腺苷（8-Br-cyclic AMP）和佛波醇12,13-二丁酸酯（PDBu）对培养的小鼠背根神经节神经元3种电压依赖性钙电流成分的影响。毛喉素和8-溴环磷酸腺苷选择性地降低了瞬时高阈值（N型）钙电流成分，蛋白激酶C激活剂PDBu也产生了同样的效果。用百日咳毒素预处理培养物可阻止PDBu对钙电流的降低作用，但对毛喉素或8-溴环磷酸腺苷引起的钙电流降低没有影响。这些结果支持以下观点：A激酶和蛋白激酶C的激活均通过降低N型钙电流成分选择性地影响脊椎动物神经元中的钙电流。虽然蛋白激酶C的激活需要G蛋白发挥作用，但A激酶的激活则不需要。
