Cha Choong-Hwan, An Hae-Kyong, Kim Jeong-Uk
Department of Laboratory Medicine, Gangneung Asan Hospital, University of Ulsan College of Medicine, 415, Bangdong-ri, Sacheon-myeon, Gangeung-si, Gangwon-do, 210-711, Korea.
Department of Laboratory Medicine, Gangneung Asan Hospital, University of Ulsan College of Medicine, 415, Bangdong-ri, Sacheon-myeon, Gangeung-si, Gangwon-do, 210-711, Korea.
Diagn Microbiol Infect Dis. 2014 May;79(1):49-53. doi: 10.1016/j.diagmicrobio.2014.01.009. Epub 2014 Jan 24.
There are few commercial assays that easily and correctly identify the mycobacteria from culture in a clinical laboratory with a high workload. Thus, we developed and evaluated a scheme for the identification of mycobacteria using a multiplex real-time PCR assay and report on its application in our laboratory. The scheme consisted of 3 stepwise PCRs. Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM) were differentially detected in the step 1 PCR, and the NTM species were identified in the step 2 and 3 PCRs. Over the 1.5-year study period, 1136 isolates of MTC and 618 isolates of NTM were detected, and the species of 608 (98.4%) of the 618 NTM isolates were identified. We conclude that the established scheme is a very useful diagnostic approach for the rapid and accurate identification of MTC and clinically relevant NTM in a clinical laboratory in a region where tuberculosis is endemic.
在工作量较大的临床实验室中，能够轻松且正确地从培养物中鉴定出分枝杆菌的商业检测方法很少。因此，我们开发并评估了一种使用多重实时PCR检测法鉴定分枝杆菌的方案，并报告了其在我们实验室中的应用情况。该方案由3个逐步进行的PCR组成。在第1步PCR中对结核分枝杆菌复合群（MTC）和非结核分枝杆菌（NTM）进行差异检测，在第2步和第3步PCR中鉴定NTM的种类。在为期1.5年的研究期间，共检测到1136株MTC菌株和618株NTM菌株，618株NTM菌株中的608株（98.4%）被鉴定出种类。我们得出结论，在结核病流行地区的临床实验室中，所建立的方案是一种非常有用的诊断方法，可用于快速准确地鉴定MTC和临床相关的NTM。
