Hailfinger Stephan, Pelzer Christiane, Thome Margot
Department of Biochemistry, University of Lausanne, Lausanne, Switzerland.
Methods Mol Biol. 2014;1133:177-88. doi: 10.1007/978-1-4939-0357-3_11.
The paracaspase MALT1 is a Cys-dependent, Arg-specific protease that plays an essential role in the activation and proliferation of lymphocytes during the immune response. Oncogenic activation of MALT1 is associated with the development of specific forms of B-cell lymphomas. Through specific cleavage of its substrates, MALT1 controls various aspects of lymphocyte activation, including the activation of transcriptional pathways, the stabilization of mRNAs, and an increase in cellular adhesion. In lymphocytes, the activity of MALT1 is tightly controlled by its inducible monoubiquitination, which promotes the dimerization of MALT1. Here, we describe both in vitro and in vivo assays that have been developed to assess MALT1 activity.
旁胱天蛋白酶MALT1是一种依赖半胱氨酸、作用于精氨酸的特异性蛋白酶，在免疫反应期间淋巴细胞的激活和增殖中发挥着重要作用。MALT1的致癌激活与特定形式的B细胞淋巴瘤的发生有关。通过对其底物的特异性切割，MALT1控制淋巴细胞激活的各个方面，包括转录途径的激活、mRNA的稳定以及细胞黏附的增加。在淋巴细胞中，MALT1的活性受到其可诱导的单泛素化的严格控制，这种单泛素化促进了MALT1的二聚化。在此，我们描述了已开发出的用于评估MALT1活性的体外和体内试验。
