异常维吾尔药赛维丹总酚联合化疗药物诱导人宫颈癌细胞凋亡的机制
Mechanisms of hela cell apoptosis induced by abnormal Savda Munziq total phenolics combined with chemotherapeutic agents.
作者信息
Zhang Yun-Xia, Abliz Guzalnur, Ye Wei-Jun, Mutalipu Zuohelaguli, Li Xiao-Wen, Wang Hai-Qin, Buranjiang Gulimire, Upur Halmurat
机构信息
The 6th Department of Gynecology, the Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China E-mail :
出版信息
Asian Pac J Cancer Prev. 2014;15(2):743-7. doi: 10.7314/apjcp.2014.15.2.743.
OBJECTIVE
To investigate the effects of abnormal Savda Munziq (ASMq) total phenolics combined with cisplatin and docetaxel on the Hela cell growth.
METHODS
In vivo cultured Hela cells were treated with cisplatin, docetaxel, total phenolics, cisplatin+total phenolics or docetaxel+total phenolics. MTT was performed to assess inhibition of cell proliferation, flow cytometry to detect apoptosis, and semi-quantitative RT-PCR to test for survivin and Bcl-2 expression.
RESULTS
The total phenolics, cisplatin and docetaxel had significant inhibitory and apoptosis-promoting effects on Hela cells (P<0.05), with the early apoptotic rates of 12.8±0.70%, 18.9±3.79% and 15.8±3.8)%; the total phenolics, cisplatin and docetaxel significantly decreased the expression of Bcl-2 and survivin (all P<0.01), especially when used in combination.
CONCLUSION
ASMq total phenolics, combined with cisplatin and docetaxel, could promote the apoptosis of Hela cells possibly through reducing the expression of Bcl-2 and survivin.
目的
探讨异常黑胆质成熟剂总酚联合顺铂和多西他赛对人宫颈癌Hela细胞生长的影响。
方法
将体外培养的Hela细胞分别用顺铂、多西他赛、总酚、顺铂+总酚或多西他赛+总酚处理。采用MTT法检测细胞增殖抑制率，流式细胞术检测细胞凋亡率，半定量RT-PCR检测生存素和Bcl-2的表达。
结果
总酚、顺铂和多西他赛对Hela细胞均有显著的抑制增殖和促进凋亡作用（P<0.05），早期凋亡率分别为12.8±0.70%、18.9±3.79%和15.8±3.8%；总酚、顺铂和多西他赛均能显著下调Bcl-2和生存素的表达（均P<0.01），联合应用时作用更明显。
结论
异常黑胆质成熟剂总酚联合顺铂和多西他赛可能通过下调Bcl-2和生存素的表达促进Hela细胞凋亡。
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