Poot M, Schindler D, Kubbies M, Hoehn H, Rabinovitch P S
Department of Human Genetics, University of Würzburg, Federal Republic of Germany.
Cytometry. 1988 Jul;9(4):332-8. doi: 10.1002/cyto.990090410.
The BrdUrd-Hoechst method was used to analyze the interaction of various oxygen concentrations with BrdUrd substituted DNA with respect to cellular proliferation. At oxygen concentrations above 5%, human diploid fibroblast-like cells and amniotic fluid fibroblast-like cells showed reduced proliferation rates, which resulted from an increase in noncycling cells and from a permanent arrest of cells in the G2 phase of the cell cycle. At 35% oxygen the increase in noncyling cell fraction and the permanent arrest in G2 was strongly dependent upon the concentration of BrdUrd. Incorporation of BrdUrd into DNA, therefore, amplifies the adverse effects of increasing oxygen concentrations upon cell proliferation. The mechanism of this amplification might involve a free radical attack on DNA similar to the radiation sensitizing effect of BrdUrd.
采用溴脱氧尿苷-赫斯特方法分析了不同氧浓度与溴脱氧尿苷取代的DNA在细胞增殖方面的相互作用。在氧浓度高于5%时，人二倍体成纤维样细胞和羊水成纤维样细胞的增殖速率降低，这是由于非循环细胞增加以及细胞在细胞周期的G2期永久停滞所致。在35%氧浓度下，非循环细胞比例的增加和G2期的永久停滞强烈依赖于溴脱氧尿苷的浓度。因此，溴脱氧尿苷掺入DNA会放大氧浓度增加对细胞增殖的不利影响。这种放大机制可能涉及自由基对DNA的攻击，类似于溴脱氧尿苷的辐射增敏作用。
