Jin Xuelong, Chen Zequn, Liu Xingju, Liang Bin, Zhang Hong, Zhang Zhiwen
Department of Physiology & Pathophysiology, Tianjin Medical University, Tianjin 300070, China.
Department of Physiology & Pathophysiology, Tianjin Medical University, Tianjin 300070, China.
Brain Res. 2014 Apr 16;1558:84-9. doi: 10.1016/j.brainres.2014.02.032. Epub 2014 Feb 22.
To visualize the dynamic expression of endothelial barrier antigen (EBA) and S100B in the rat parietal cortex at the acute phase of radiation-induced brain injury using computed tomography (CT).
A rat model of brain injury was established by CT scanning. The expression of EBA and S100B in the parietal cortex was analyzed at different time points by immunohistochemistry (IHC) and western blotting.
Significantly increased EBA expression was detected in the animals in the control group compared with the animals receiving CT radiation, which exhibited significantly reduced EBA levels within the vessel walls (F=33.29, p<0.05), particularly at day 3 after radiation. Both immunohistochemical staining and western blot analysis indicated that the positive expression levels of S100B among radiation groups were increased compared with the control group (IHC, F=28.05, p<0.05; WB, F=175.3, p<0.05). The expression of S100B peaked at day 3 (IHC, 102718±8710; WB, 2320±0.129), and subsequently decreased.
CT radiation can induce altered EBA and S100B protein expression. Decreased EBA expression levels indicated that the integrity of the blood-brain barrier (BBB) was affected by radiation. The destruction of the BBB and the expression of S100B might play important roles in the incidence and repair of the early radiation-induced brain injury, and radiation represents a cause of mental disorders.
利用计算机断层扫描（CT）观察大鼠放射性脑损伤急性期顶叶皮质中内皮屏障抗原（EBA）和S100B的动态表达。
通过CT扫描建立大鼠脑损伤模型。采用免疫组织化学（IHC）和蛋白质印迹法在不同时间点分析顶叶皮质中EBA和S100B的表达。
与接受CT辐射的动物相比，对照组动物的EBA表达显著增加，接受CT辐射的动物血管壁内EBA水平显著降低（F=33.29，p<0.05），尤其是在辐射后第3天。免疫组织化学染色和蛋白质印迹分析均表明，与对照组相比，辐射组中S100B的阳性表达水平升高（免疫组织化学，F=28.05，p<0.05；蛋白质印迹，F=175.3，p<0.05）。S100B的表达在第3天达到峰值（免疫组织化学，102718±8710；蛋白质印迹，2320±0.129），随后下降。
CT辐射可诱导EBA和S100B蛋白表达改变。EBA表达水平降低表明血脑屏障（BBB）的完整性受到辐射影响。血脑屏障的破坏和S100B的表达可能在早期放射性脑损伤的发生和修复中起重要作用，且辐射是精神障碍的一个病因。
