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PTRN-1是一种微管负端结合的CAMSAP同源物，可促进秀丽隐杆线虫神经元中的微管功能。

PTRN-1, a microtubule minus end-binding CAMSAP homolog, promotes microtubule function in Caenorhabditis elegans neurons.

作者信息

Richardson Claire E, Spilker Kerri A, Cueva Juan G, Perrino John, Goodman Miriam B, Shen Kang

机构信息

Department of Biology, Stanford University, Stanford, United States.

出版信息

Elife. 2014 Feb 25;3:e01498. doi: 10.7554/eLife.01498.

DOI:10.7554/eLife.01498
PMID:24569477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3932522/
Abstract

In neuronal processes, microtubules (MTs) provide structural support and serve as tracks for molecular motors. While it is known that neuronal MTs are more stable than MTs in non-neuronal cells, the molecular mechanisms underlying this stability are not fully understood. In this study, we used live fluorescence microscopy to show that the C. elegans CAMSAP protein PTRN-1 localizes to puncta along neuronal processes, stabilizes MT foci, and promotes MT polymerization in neurites. Electron microscopy revealed that ptrn-1 null mutants have fewer MTs and abnormal MT organization in the PLM neuron. Animals grown with a MT depolymerizing drug caused synthetic defects in neurite branching in the absence of ptrn-1 function, indicating that PTRN-1 promotes MT stability. Further, ptrn-1 null mutants exhibited aberrant neurite morphology and synaptic vesicle localization that is partially dependent on dlk-1. Our results suggest that PTRN-1 represents an important mechanism for promoting MT stability in neurons. DOI: http://dx.doi.org/10.7554/eLife.01498.001.

摘要

在神经元突起中，微管（MTs）提供结构支撑并作为分子马达的轨道。虽然已知神经元微管比非神经元细胞中的微管更稳定，但其稳定性背后的分子机制尚未完全了解。在本研究中，我们使用实时荧光显微镜显示秀丽隐杆线虫的CAMSAP蛋白PTRN-1定位于沿神经元突起的斑点，稳定微管焦点，并促进神经突中的微管聚合。电子显微镜显示ptrn-1基因敲除突变体在PLM神经元中的微管较少且微管组织异常。在缺乏ptrn-1功能的情况下，用微管解聚药物培养的动物在神经突分支中导致合成缺陷，表明PTRN-1促进微管稳定性。此外，ptrn-1基因敲除突变体表现出异常的神经突形态和突触小泡定位，这部分依赖于dlk-1。我们的结果表明，PTRN-1代表了促进神经元中微管稳定性的重要机制。DOI：http://dx.doi.org/10.7554/eLife.01498.001。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd3f/3932522/36ee4c472e90/elife01498f008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd3f/3932522/b47319487dfb/elife01498fs010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd3f/3932522/26d3f05b4a44/elife01498f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd3f/3932522/779b07151ae5/elife01498fs011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd3f/3932522/6096029811f7/elife01498f006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd3f/3932522/36ee4c472e90/elife01498f008.jpg

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