Skrzypczak Michał, Rokicki Jerzy, Pawliczka Iwona, Najda Katarzyna, Dzido Joanna
Department of Invertebrate Zoology and Parasitology, Laboratory of Parasitology and General Zoology, University of Gdansk, Faculty of Biology, Wita Stwosza 59, 80-308, Gdańsk, Poland.
Acta Parasitol. 2014 Mar;59(1):165-72. doi: 10.2478/s11686-014-0226-2. Epub 2014 Feb 26.
In the present study 5 grey seals (Halichoerus grypus), 3 common seals (Phoca vitulina) and 1 ringed seal (Pusa hispida) bycaught or stranded on the Polish Baltic Sea coast in years 2000-2006 were investigated for the infestation of parasitic anisakid nematodes. 749 of anisakids were found. The most common were: Contracaecum osculatum (59.3%) and Pseudoterranova decipiens (31.0%). There were also small numbers of Anisakis simplex (0.8%). After performing RFLP three sibling species were found. C. osculatum was identified as C. osculatum C, P decipiens was identified as P. decipiens sensu stricto and A. simplex - A. simplex sensu stricto. Nematodes found in seals were mostly in L4 and adult life stage - both of them were equal with some minor variations among the specimens. Sex ratio was also equal, but there was slight excess of males in some cases. There was a minority of L3 larvae belonging to A. simplex species (0.8%).
在本研究中,对2000年至2006年间在波兰波罗的海沿岸误捕或搁浅的5只灰海豹(Halichoerus grypus)、3只斑海豹(Phoca vitulina)和1只环斑海豹(Pusa hispida)进行了寄生异尖线虫感染情况调查。共发现749条异尖线虫。最常见的是:吻状对盲囊线虫(Contracaecum osculatum,占59.3%)和欺骗新蛔线虫(Pseudoterranova decipiens,占31.0%)。也有少量简单异尖线虫(Anisakis simplex,占0.8%)。进行限制性片段长度多态性分析(RFLP)后,发现了三个姐妹种。吻状对盲囊线虫被鉴定为吻状对盲囊线虫C型,欺骗新蛔线虫被鉴定为狭义欺骗新蛔线虫,简单异尖线虫被鉴定为狭义简单异尖线虫。在海豹体内发现的线虫大多处于L4期和成虫期——两者数量相当,不同样本之间有一些细微差异。性别比例也相当,但在某些情况下雄性略有过剩。属于简单异尖线虫种的L3幼虫占少数(0.8%)。