Elabiad Mohamad T, Arheart Kristopher L, Korones Sheldon B, Pourcyrous Massroor
Division of Neonatology, Department of Pediatrics, University of Tennessee Health Science Center (UTHSC), Memphis, Tennessee.
Division of Biostatistics and Pediatrics, Department of Epidemiology and Public Health, Miller School of Medicine, University of Miami, Miami, Florida.
Am J Perinatol. 2014 Nov;31(10):851-4. doi: 10.1055/s-0033-1361938. Epub 2014 Feb 25.
Low total serum protein levels may cause a positive bias on C-reactive protein (CRP) detected by the Vitros 250 Chemistry System (Ortho-Clinical Diagnostics, Inc., Johnson & Johnson Co., Raritan, NJ). Low total serum protein levels are observed in some infants. Our objective was to define a cutoff value for normal levels of CRP measured on the Vitros System that is comparable to the cutoff value of 1.0 mg/dL measured by rate nephelometry on a Beckman Array System (Beckman Instruments Inc., Fullerton, CA).
CRP was prospectively measured on the same serum sample on Vitros and Beckman systems. Using a result of ≥1.0 as the "gold standard" definition of an abnormal CRP, measures of association were calculated.
CRP was measured in 981 blood samples that were collected from 361 infants. A cutoff CRP level using the Vitros system at 1.5 mg/dL had the highest sensitivity and negative predictive value comparable to 1.0 mg/dL measured by nephelometry. By regression analysis, each increase by 1 mg/dL by nephelometry caused an increase by 1.5 mg/dL on the Vitros system (R(2) = 0.94; p < 0.001; slope = 0.66; 95% confidence intervals, 0.65, 0.67).
In infants, when measuring CRP levels by Vitros CRP slide system, a normal reference level of 1.5 mg/dL instead of 1 mg/dL should be used.
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