Department of Poultry Science, Mississippi State University, Mississippi State 39762.
Poult Sci. 2014 Jan;93(1):155-62. doi: 10.3382/ps.2013-03389.
In ovo injection of 25-hydroxycholecalciferol [25(OH)D3] has been shown to improve hatchability of injected broiler eggs. Effects of the in ovo injection of commercial diluent containing various levels of 25(OH)D3 on broiler posthatch performance and carcass characteristics were investigated in 2 trials. On each of 10 tray levels of a single-stage incubator, all treatment groups were randomly represented with each containing 21 and 40 eggs in trials 1 and 2, respectively. Treatments included noninjected and diluent-injected (100 µL commercial diluent-injected) controls, and those that received 0.15, 0.30, 0.60, or 1.20 µg of 25(OH)D3 in trial 1 and 0.20, 0.60, 1.80, or 5.4 µg of 25(OH)D3 in trial 2, in 100 µL of commercial diluent using a commercial multi-egg injector on d 18 of incubation. On d 21 of incubation, chicks from each treatment replicate group were placed in corresponding floor pens. Feed intake, BW gain, and feed conversion were determined for the weekly and cumulative 0 to 21 d age intervals in trial 1 and for the weekly and cumulative 0 to 48 d age intervals in trial 2. In trial 2, 2 birds from each sex were selected from each treatment replicate group for determination of processing carcass characteristics on d 49 posthatch. Feed intake in the 0 to 7, 7 to 14, and 14 to 21 d age intervals and BW gain in the 0 to 7 and 14 to 21 d age intervals were affected by treatment in trial 2. The in ovo injection of up to 1.20 and 0.60 µg of 25(OH)D3 in trials 1 and 2, respectively, did not affect the BW gain of birds during the 0 to 7, 7 to 14, and 14 to 21 d age intervals compared with the noninjected and diluent-injected control groups. It was concluded that the in ovo injection of up to 1.20 µg of 25(OH)D3 into the amnion has no detrimental effect on the overall posthatch performance of broilers.
在鸡胚中注射 25-羟胆钙化醇[25（OH）D3]已被证明可以提高注射肉鸡鸡蛋的孵化率。本试验研究了在鸡胚中注射含有不同水平 25（OH）D3 的商业稀释剂对肉鸡出雏后性能和屠体特征的影响。在单层孵化器的 10 个托盘水平中的每一个上，所有处理组均随机代表，试验 1 中每个处理组包含 21 个和 40 个鸡蛋，试验 2 中每个处理组包含 21 个和 40 个鸡蛋。处理组包括非注射和稀释剂注射（100 µL 商业稀释剂注射）对照组，以及在试验 1 中接受 0.15、0.30、0.60 或 1.20 µg 25（OH）D3，在试验 2 中接受 0.20、0.60、1.80 或 5.4 µg 25（OH）D3 的组，每个处理组均在 18 日龄时用商业多蛋注射器注射 100 µL 商业稀释剂。在孵化第 21 天，每个处理组的雏鸡被放置在相应的地板围栏中。在试验 1 中，在 0 至 21 日龄的每周和累计年龄间隔以及在试验 2 中，在 0 至 48 日龄的每周和累计年龄间隔中确定了饲料摄入量、体重增加和饲料转化率。在试验 2 中，从每个处理组的每个性别中选择 2 只鸡，以确定孵化后第 49 天的屠宰体特征。在试验 2 中，处理组影响 0 至 7、7 至 14 和 14 至 21 日龄的饲料摄入量和 0 至 7 和 14 至 21 日龄的体重增加。在试验 1 和 2 中，向羊膜内注射高达 1.20 和 0.60 µg 的 25（OH）D3 不会影响与未注射和稀释剂注射对照组相比，0 至 7、7 至 14 和 14 至 21 日龄期间的雏鸡体重增加。因此，向羊膜内注射高达 1.20 µg 的 25（OH）D3 对肉鸡出雏后的整体性能没有不良影响。
