Loretz C A, Fourtner C R
Department of Biological Sciences, State University of New York, Buffalo 14260.
J Exp Biol. 1988 May;136:383-403. doi: 10.1242/jeb.136.1.383.
An anion channel was isolated, using patch-clamp technique, from the basolateral membrane of goby intestinal epithelial cells. Single-channel conductance varied over a range from 20 to 90 pS. The channel was voltage-gated over the physiological range of cell membrane potential with depolarization increasing the proportion of time in the open state. There was no Ca2+ sensitivity. The selectivity sequence was SO4(2-) greater than Cl- greater than Mes-. The channel may function in vivo as one of several avenues of basolateral membrane Cl- exit with the voltage-gating property serving to match basolateral Cl- exit to apical entry.
利用膜片钳技术，从虎鱼肠道上皮细胞的基底外侧膜分离出一种阴离子通道。单通道电导在20至90皮西门子范围内变化。该通道在细胞膜电位的生理范围内是电压门控的，去极化增加了开放状态的时间比例。没有Ca2+敏感性。选择性顺序为SO4(2-)大于Cl-大于Mes-。该通道在体内可能作为基底外侧膜Cl-流出的几种途径之一发挥作用，其电压门控特性有助于使基底外侧Cl-流出与顶端进入相匹配。
