Samejima N, Yamazaki K
First Department of Surgery, Asahikawa Medical College, Japan.
Jpn J Surg. 1988 May;18(3):235-42. doi: 10.1007/BF02471439.
In order to study the vascular proliferation in human breast cancer, blood vessels were counted, per square millimeter, in the tissue immediately around tumors. Mastectomized specimens of 84 patients with breast cancer and specimens from 10 patients with benign mammary diseases were stained by hematoxylin eosin and, where required, by the avidin biotin peroxidase complex method for laminin staining. The vascular density around the breast cancer tissue was 20.35 +/- 8.40/mm2, which was significantly higher than the value of 13.44 +/- 5.85/mm2 for noncancerous mammary tissues (p less than 0.001) or the value of 12.65 +/- 4.12/mm2 for benign mammary disease tissues (p less than 0.01). Among the breast cancers, noninvasive carcinoma had a higher vascular density (28.44 +/- 6.15/mm2) than invasive carcinoma (19.73 +/- 8.22/mm2, p less than 0.02). According to the Japan Mammary Cancer Society Classification of invasive ductal carcinoma, vascularity was higher in the papillotubular type of cancer than in the solid-tubular or scirrhous types of cancer (p less than 0.02), although the papillotubular type had the lowest rate of nodal metastasis and vascular invasion as compared with the scirrhous and solid-tubular types. The vascular density around the tumors did not change in association with an increase in tumor size and it was suggested that blood vessels around a tumor would increase almost in proportion to the square of the tumor diameter.
为研究人类乳腺癌中的血管增殖情况，对肿瘤周围紧邻组织每平方毫米的血管进行计数。84例乳腺癌患者的乳房切除标本以及10例乳腺良性疾病患者的标本用苏木精伊红染色，必要时用抗生物素蛋白 - 生物素过氧化物酶复合物法进行层粘连蛋白染色。乳腺癌组织周围的血管密度为20.35±8.40/mm²，显著高于非癌性乳腺组织的13.44±5.85/mm²（p<0.001）或乳腺良性疾病组织的12.65±4.12/mm²（p<0.01）。在乳腺癌中，非浸润性癌的血管密度（28.44±6.15/mm²）高于浸润性癌（19.73±8.22/mm²，p<0.02）。根据日本乳腺癌协会浸润性导管癌分类，乳头状管状型癌的血管分布高于实体管状型或硬癌型癌（p<0.02），尽管与硬癌型和实体管状型相比，乳头状管状型的淋巴结转移率和血管侵犯率最低。肿瘤周围的血管密度不随肿瘤大小增加而变化，提示肿瘤周围血管增加量几乎与肿瘤直径的平方成正比。
