Jing Lizhong, Zhang Jiying, Leng Huijie, Guo Qinwei, Hu Yuelin
Institute of Sports Medicine, Peking University Third Hospital, Beijing, 100191, People's Republic of China.
Knee Surg Sports Traumatol Arthrosc. 2015 Apr;23(4):1119-27. doi: 10.1007/s00167-014-2906-8. Epub 2014 Feb 27.
To demonstrate that iliac crest cartilage may be used to repair articular cartilage defects in the knees of rabbits.
Full-thickness cartilage defects were created in the medial femoral condyle on both knees of 36 New Zealand white rabbits. The 72 defects were randomly assigned to be repaired with ipsilateral iliac crest cartilage (Group I), osteochondral tissues removed at defect creation (Group II), or no treatment (negative control, Group III). Animals were killed at 6, 12, and 24 weeks post-operatively. The repaired tissues were harvested for magnetic resonance imaging (MRI), histological studies (haematoxylin and eosin and immunohistochemical staining), and mechanical testing.
At 6 weeks, the iliac crest cartilage graft was not yet well integrated with the surrounding articular cartilage, but at 12 weeks, the graft deep zone had partial ossification. By 24 weeks, the hyaline cartilage-like tissue was completely integrated with the surrounding articular cartilage. Osteochondral autografts showed more rapid healing than Group I at 6 weeks and complete healing at 12 weeks. Untreated defects were concave or partly filled with fibrous tissue throughout the study. MRI showed that Group I had slower integration with surrounding normal cartilage compared with Group II. The mechanical properties of Group I were significantly lower than those of Group II at 12 weeks, but this difference was not significant at 24 weeks.
Iliac crest cartilage autografts were able to repair knee cartilage defects with hyaline cartilage and showed comparable results with osteochondral autografts in the rabbit model.
证明髂嵴软骨可用于修复兔膝关节的关节软骨缺损。
在36只新西兰白兔的双膝内侧股骨髁制造全层软骨缺损。将72处缺损随机分配，分别采用同侧髂嵴软骨修复（第一组）、缺损创建时移除的骨软骨组织修复（第二组）或不进行治疗（阴性对照组，第三组）。术后6周、12周和24周处死动物。采集修复组织用于磁共振成像（MRI）、组织学研究（苏木精和伊红染色及免疫组织化学染色）和力学测试。
6周时，髂嵴软骨移植物与周围关节软骨尚未良好整合，但12周时，移植物深层区域出现部分骨化。到24周时，透明软骨样组织已与周围关节软骨完全整合。骨软骨自体移植在6周时比第一组愈合更快，在12周时完全愈合。在整个研究过程中，未治疗的缺损呈凹陷状或部分充满纤维组织。MRI显示，与第二组相比，第一组与周围正常软骨的整合较慢。第一组的力学性能在12周时显著低于第二组，但在24周时这种差异不显著。
在兔模型中，髂嵴软骨自体移植能够用透明软骨修复膝关节软骨缺损，且结果与骨软骨自体移植相当。
