Krumwieh D, Schulz G, Seiler F R
Research Laboratories of Behringwerke AG, Marburg/Lahn, W.-Germany.
Behring Inst Mitt. 1988 Apr(82):265-81.
Proliferation and differentiation of stem cells within the bone marrow into different cell types circulating in the peripheral blood is regulated by a series of hierarchically acting growth mediators--the colony-stimulating factors. In recent years, the genes of some of these physiological glycoproteins were cloned by means of molecular biology, and thus highly purified recombinant protein can now be produced in large scale. This has provided access to profound investigation of the role of these factors and the mechanisms for their action with regard to their potential therapeutical use. Part of their properties as revealed by the various in vitro test systems and by corresponding efficacy models using laboratory animals could also be found in recently performed phase I clinical studies, i.e. for granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF). Perspectives for a potential use of these regulators as single therapy and in possible additive or synergistic combinations are currently intensively studied.
骨髓内干细胞增殖并分化为外周血中循环的不同细胞类型，这一过程受一系列具有层级作用的生长介质——集落刺激因子调控。近年来，借助分子生物学手段克隆了其中一些生理性糖蛋白的基因，因而现在能够大规模生产高度纯化的重组蛋白。这为深入研究这些因子的作用及其作用机制在潜在治疗用途方面的应用提供了途径。通过各种体外测试系统以及使用实验动物的相应疗效模型所揭示的这些因子的部分特性，在最近进行的I期临床研究中也有发现，即粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）。目前正在深入研究将这些调节剂作为单一疗法以及可能的联合或协同组合使用的潜在前景。
