茶碱对两栖动物皮肤顶端钠和氯通透性的影响。
Effect of theophylline on the apical sodium and chloride permeabilities of amphibian skin.
作者信息
Katz U, Van Driessche W
机构信息
Department of Biology Technion, Haifa, Israel.
出版信息
J Physiol. 1988 Mar;397:223-36. doi: 10.1113/jphysiol.1988.sp016997.
Abstract
- The effects of theophylline (1 mmol/l) on the sodium transport (short-circuit current, Isc) and transepithelial conductance (Gtotal) through toad (Bufo viridis) and frog (Rana temporaria and Rana esculenta) skin were investigated. 2. In toad skin incubated with nitrate Ringer solution on the apical side, theophylline induced an increase in Isc similar to that in frog skin bathed with chloride Ringer solution. 3. The increase in Isc could be attributed to recruitment of sodium channels, without affecting the single-channel current. 4. Chloride-bathed toad skin responded to theophylline with a large increase in transepithelial conductance, in addition to the increased Isc. 5. Chloride replacement by nitrate eliminated the effect of theophylline on the conductance increase, but the Isc response was even larger. 6. The results are discussed in relation to the localization of the cellular chloride pathway to the mitochondria-rich cells.
摘要
- 研究了氨茶碱（1毫摩尔/升）对蟾蜍（绿蟾蜍）和青蛙（林蛙和食用蛙）皮肤钠转运（短路电流，Isc）和跨上皮电导（Gtotal）的影响。2. 在顶端侧用硝酸盐林格溶液孵育的蟾蜍皮肤中，氨茶碱引起的Isc增加类似于用氯化物林格溶液浸泡的青蛙皮肤中的情况。3. Isc的增加可归因于钠通道的募集，而不影响单通道电流。4. 除了Isc增加外，用氯化物浸泡的蟾蜍皮肤对氨茶碱的反应是跨上皮电导大幅增加。5. 用硝酸盐替代氯化物消除了氨茶碱对电导增加的影响，但Isc反应更大。6. 结合细胞氯化物途径定位于富含线粒体的细胞这一情况对结果进行了讨论。
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