Boismenu R, Dubow M S, Murgita R A
Department of Microbiology and Immunology, McGill University, Montreal, Canada.
Life Sci. 1988;43(8):673-81. doi: 10.1016/0024-3205(88)90138-5.
A mouse alpha-fetoprotein complementary DNA containing the coding region for amino acids 256 to 548 was fused to the lac transcriptional and translational control elements contained on the expression vector pOP203-28. The expression of a 35 kD hybrid lac Z'- alpha-fetoprotein polypeptide in Escherichia coli was demonstrated by the chloramphenicol release assay and by immunoprecipitation using rabbit anti-mouse alpha-fetoprotein antibodies as probe.
将包含氨基酸256至548编码区的小鼠甲胎蛋白互补DNA与表达载体pOP203 - 28上的乳糖操纵子转录和翻译控制元件融合。通过氯霉素释放试验以及使用兔抗小鼠甲胎蛋白抗体作为探针进行免疫沉淀，证明了在大肠杆菌中35kD杂合乳糖Z'-甲胎蛋白多肽的表达。
