己酮可可碱对大鼠尿道梗阻后肾脏结构的影响：一项体视学研究
Effects of pentoxifylline on renal structure after urethral obstruction in rat: A stereological study.
作者信息
Shirazi Mehdi, Noorafshan Ali, Farrokhi Amir
机构信息
Histomorphometry & Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Urology, Shahid Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
Cent European J Urol. 2011;64(1):30-3. doi: 10.5173/ceju.2011.01.art6. Epub 2011 Mar 18.
OBJECTIVE
Chronic renal failure due to tubulointerstitial fibrosis is one of complications of lower urinary tract obstruction. Since pentoxifylline is a xanthine derivative that inhibits some inflammatory mediators, we conducted this study to investigate whether pentoxifylline inhibits renal fibrosis in a rat model of partial urethral obstruction (PUO).
METHODS
All the rats underwent experimental PUO. Then, the animals were divided randomly into two groups: positive control group and experimental group. The experimental group received pentoxifylline 100 mg/kg per day via oral gavages for 4-weeks. The control group received the same dose of normal saline. After 4-weeks, all the rats underwent left nephrectomy. Kidney volume and weight and fractional and absolute volumes of the glomeruli, tubules, interstitium and vessels were determined with stereological methods. To reduce the workload of reference (kidney) volume estimation, the total kidney volume was determined after estimation of tissue shrinkage on isotropic uniform random histological sections. The total volume (amount) of each renal structure including fibrosis was estimated to avoid the bias conclusion due to relying on volume density alone.
RESULTS
The absolute volume of interstitial fibrosis was lower in the experimental group (PUO with pentoxifylline treatment) (~84%; p ≤0.006) in comparison with the control group (PUO with no treatment).
CONCLUSION
Pentoxifylline reduces interstitial renal fibrosis after partial urethral obstruction in rats.
目的
肾小管间质纤维化所致的慢性肾衰竭是下尿路梗阻的并发症之一。由于己酮可可碱是一种抑制某些炎症介质的黄嘌呤衍生物，我们开展了本研究，以探讨己酮可可碱是否能在大鼠部分尿道梗阻（PUO）模型中抑制肾纤维化。
方法
所有大鼠均接受实验性PUO。然后，将动物随机分为两组：阳性对照组和实验组。实验组每天经口灌胃给予己酮可可碱100mg/kg，持续4周。对照组给予相同剂量的生理盐水。4周后，所有大鼠均接受左肾切除术。采用体视学方法测定肾脏体积、重量以及肾小球、肾小管、间质和血管的分数体积和绝对体积。为减少参考（肾脏）体积估计的工作量，在各向同性均匀随机组织学切片上估计组织收缩后测定总肾脏体积。估计每个肾脏结构（包括纤维化）的总体积（数量），以避免仅依赖体积密度得出有偏差的结论。
结果
与对照组（未治疗的PUO）相比，实验组（己酮可可碱治疗的PUO）间质纤维化的绝对体积较低（约84%；p≤0.006）。
结论
己酮可可碱可减轻大鼠部分尿道梗阻后的肾间质纤维化。
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