Laboratory of Molecular Biology, Department of Microbiology and Immunology, College of Medicine, University of South Alabama, 501 Aubrey Green Drive, Mobile, AL 36688-0002, United States.
Laboratory of Molecular Biology, Department of Microbiology and Immunology, College of Medicine, University of South Alabama, 501 Aubrey Green Drive, Mobile, AL 36688-0002, United States.
Curr Opin Microbiol. 2014 Feb;17:11-6. doi: 10.1016/j.mib.2013.10.006. Epub 2013 Dec 6.
Rapid advancements in the genetic manipulation of obligate intracellular bacterial pathogens have been made over the past two years. In this paper we attempt to summarize the work published since 2011 that documents these exciting accomplishments. Although each genus comprising this diverse group of pathogens poses unique problems, requiring modifications of established techniques and the introduction of new tools, all appear amenable to genetic analysis. Significantly, the field is moving forward from a focus on the identification and development of genetic techniques to their application in addressing crucial questions related to mechanisms of bacterial pathogenicity and the requirements of obligate intracellular growth.
在过去的两年中,人们在对专性细胞内细菌病原体进行基因操作方面取得了快速进展。在本文中,我们试图总结自 2011 年以来发表的这些激动人心的研究成果。尽管该病原体群体中的每个属都带来了独特的问题,需要对现有技术进行修改并引入新的工具,但所有属似乎都适用于遗传分析。重要的是,该领域正在从专注于遗传技术的鉴定和开发,转向将其应用于解决与细菌致病性机制和专性细胞内生长要求相关的关键问题。