Ibrahim Mohammed Auwal, Islam M Shahidul
Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Durban 4000, South Africa; Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Durban 4000, South Africa.
J Ethnopharmacol. 2014 Apr 28;153(2):392-9. doi: 10.1016/j.jep.2014.02.042. Epub 2014 Feb 26.
Senna singueana is currently used in the traditional treatment of diabetes mellitus in Nigeria. The present study examined the anti-diabetic activity of the Senna singueana acetone fraction (SSAF) of stem bark in a type 2 diabetes (T2D) rat model.
Crude ethyl acetate extract of the Senna singueana stem bark was fractionated with various solvents and the acetone fraction was selected for in vivo studies based on the high α-glucosidase and α-amylase inhibitory activities. In the in vivo study, male Sprague-Dawley rats were induced with T2D and treated with the SSAF at 150 and 300 mg/kg body weight. Several T2D-related parameters were measured in the study.
After 4 weeks of intervention, non-fasting blood glucose concentrations were significantly decreased and the glucose tolerance ability was significantly improved in the SSAF treated groups compared to the diabetic control group. Serum insulin concentrations, pancreatic β-cell function (HOMA-β) and liver glycogen were significantly (P<0.05) increased while serum alanine transaminase, alkaline phosphatase and urea were significantly decreased in the SSAF treated diabetic rats compared to the diabetic control group. Though insignificantly (P>0.05), other T2D-induced abnormalities such as food and fluid intake, body weight, serum lipids, serum fructosamine level and peripheral insulin resistance (HOMA-IR) were also partially ameliorated by the SSAF treatment.
Data of this study suggest that orally administered SSAF could ameliorate most of the T2D-induced abnormalities in a T2D model of rats.
在尼日利亚，单叶番泻目前被用于传统的糖尿病治疗。本研究在2型糖尿病（T2D）大鼠模型中检测了单叶番泻茎皮丙酮提取物（SSAF）的抗糖尿病活性。
单叶番泻茎皮的粗乙酸乙酯提取物用各种溶剂进行分级分离，基于高α-葡萄糖苷酶和α-淀粉酶抑制活性选择丙酮提取物进行体内研究。在体内研究中，雄性Sprague-Dawley大鼠被诱导成T2D，并以150和300mg/kg体重的剂量用SSAF进行治疗。在研究中测量了几个与T2D相关的参数。
干预4周后，与糖尿病对照组相比，SSAF治疗组的非空腹血糖浓度显著降低，葡萄糖耐量能力显著提高。与糖尿病对照组相比，SSAF治疗的糖尿病大鼠血清胰岛素浓度、胰腺β细胞功能（HOMA-β）和肝糖原显著（P<0.05）增加，而血清丙氨酸转氨酶、碱性磷酸酶和尿素显著降低。虽然不显著（P>0.05），但SSAF治疗也部分改善了其他T2D诱导的异常，如食物和液体摄入量、体重、血脂、血清果糖胺水平和外周胰岛素抵抗（HOMA-IR）。
本研究数据表明，口服SSAF可改善大鼠T2D模型中大多数T2D诱导的异常。
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