Guangdong Engineering and Technology Research Center for Quality and Efficacy Reevaluation of Post-Market Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, People's Republic of China.
Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, People's Republic of China.
Sci Rep. 2020 Jul 2;10(1):10829. doi: 10.1038/s41598-020-67362-2.
Naoxintong Capsule (NXT) is a Traditional Chinese Medicine formulation which has been widely applied in treating cardiovascular and cerebrovascular diseases. Previous studies also reported the potential effects of NXT against diabetes and certain complications, yet its mechanisms remain largely obscured. Herein, in this study, we investigated the anti-diabetic effects of NXT as well as its potential mechanisms. Type 2 diabetes (T2D) was induced in rats by 10-week high-fat diet in companion with a low-dose streptozotocin injection. NXT was administrated for additional 8 weeks. The results showed that NXT exerted potent efficacy against T2D by alleviating hyperglycemia and hyperlipidemia, ameliorating insulin resistance, mitigating inflammation, relieving hypertension, and reducing myocardial injuries. To investigate its mechanisms, by integrating sequencing of gut microbiota and serum untargeted metabolomics, we showed that NXT could significantly recover the disturbances of gut microbiota and metabolic phenotypes in T2D rats. Several feature pathways, such as arachidonic acid metabolism, fatty acid β-oxidation and glycerophospholipid metabolism, were identified as the potential mechanisms of NXT in vivo. In summary, our study has comprehensively revealed the anti-diabetic effects of NXT which could be considered as a promising strategy for treating metabolic disorders, T2D and diabetic related complications in clinical practice.
脑心通胶囊（NXT）是一种中药方剂，已广泛用于治疗心脑血管疾病。先前的研究还报道了 NXT 对糖尿病和某些并发症的潜在作用，但它的作用机制仍很大程度上不清楚。在此，在这项研究中，我们研究了 NXT 的抗糖尿病作用及其潜在机制。通过 10 周高脂肪饮食联合小剂量链脲佐菌素注射诱导大鼠 2 型糖尿病（T2D）。另外给予 NXT 治疗 8 周。结果表明，NXT 通过减轻高血糖和高血脂、改善胰岛素抵抗、减轻炎症、缓解高血压和减少心肌损伤，对 T2D 具有显著疗效。为了研究其机制，我们通过整合肠道微生物组测序和血清非靶向代谢组学，表明 NXT 可显著恢复 T2D 大鼠肠道微生物组和代谢表型的紊乱。鉴定了几种特征途径，如花生四烯酸代谢、脂肪酸β-氧化和甘油磷脂代谢，作为 NXT 在体内的潜在机制。总之，我们的研究全面揭示了 NXT 的抗糖尿病作用，可考虑将其作为治疗临床实践中代谢紊乱、T2D 和糖尿病相关并发症的有前途的策略。
