Department of Laboratory Medicine, Peking University Third Hospital, Beijing, China.
Department of Urology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.
Biochem Biophys Res Commun. 2014 Mar 28;446(1):18-24. doi: 10.1016/j.bbrc.2014.01.172. Epub 2014 Feb 26.
CXCL5 (epithelial neutrophil activating peptide-78) which acts as a potent chemoattractant and activator of neutrophil function was reported to play a multifaceted role in tumorigenesis. To investigate the role of CXCL5 in bladder cancer progression, we examined the CXCL5 expression in bladder cancer tissues by real-time PCR and Western blot, additionally, we used shRNA-mediated silencing to generate stable CXCL5 silenced bladder cancer T24 cells and defined its biological functions. Our results demonstrated that mRNA and protein of CXCL5 is increased in human bladder tumor tissues and cell lines, down-regulation of CXCL5 in T24 cells resulted in significantly decreased cell proliferation, migration and increased cell apoptosis in vitro through Snail, PI3K-AKT and ERK1/2 signaling pathways. These data suggest that CXCL5 is critical for bladder tumor growth and progression, it may represent a potential application in cancer diagnosis and therapy.
趋化因子（C-X-C 基元）配体 5（epithelial neutrophil activating peptide-78，也称为 CXCL5）是一种强有力的趋化因子和中性粒细胞功能激活剂，被报道在肿瘤发生中发挥多种作用。为了研究 CXCL5 在膀胱癌进展中的作用，我们通过实时 PCR 和 Western blot 检测了膀胱癌组织中的 CXCL5 表达，此外，我们还使用 shRNA 介导的沉默生成稳定沉默 CXCL5 的膀胱癌 T24 细胞，并确定了其生物学功能。我们的结果表明，CXCL5 的 mRNA 和蛋白在人膀胱癌组织和细胞系中增加，下调 T24 细胞中的 CXCL5 导致体外细胞增殖、迁移明显减少，细胞凋亡增加，通过 Snail、PI3K-AKT 和 ERK1/2 信号通路。这些数据表明，CXCL5 对膀胱癌的生长和进展至关重要，它可能代表癌症诊断和治疗的潜在应用。
