Wang W K, Ho L T, Chiang Y, Chen T C
Institute of Physics, Academia Sinica, Taipei, Taiwan, China.
J Immunol Methods. 1988 Sep 13;112(2):173-6. doi: 10.1016/0022-1759(88)90354-7.
A metal surface has been added into a fluorometer to reflect the excited light and to reduce the light scattering. Due to the total separation of the fluorescent signal and the noise by directing them into different spaces, the resolution could be improved by several orders of magnitude. The same metal surface could also be used for immunoassays. The detection of alpha-fetoprotein in normal rat serum and human standard serum are given as examples. The results indicate that the apparatus and the method have significant advantages.
已在荧光计中添加了金属表面，以反射激发光并减少光散射。由于荧光信号和噪声被引导到不同空间而实现了完全分离，分辨率可提高几个数量级。同样的金属表面也可用于免疫测定。文中给出了正常大鼠血清和人标准血清中甲胎蛋白的检测实例。结果表明该仪器和方法具有显著优势。
